A novel mechanism of act in nucleation by nebulin complex and its physiological role

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770185
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Chiba University
• Principal Investigator: TAKANO Kazunori 千葉大学, 大学院・融合科学研究科, 助教 (60466860)
• Project Period (FY): 2010 – 2011
• Keywords: 細胞骨格 / 運動

Research Abstract

Actin filament is required for cell shape and formation of myofibril, which is contractile apparatus in striated muscle. However, the mechanism how myofibril is built during skeletal muscle hypertrophy remains obscure. In this study, not only it revealed a novel mechanism of actin filament formation at the Z-band by nebulin complex, but also the mechanism was essential for physiological skeletal muscle hypertrophy. Furthermore, this study suggested a possibility that the novel mechanism of actin filament formation might be involved in cardiac hypertrophy leading to heart failure.

Research Products

• [Journal Article] Essential roles of PACSIN2/syndapin-II in caveolae membranes culpting (2011)
  • Author(s): Senju, Y., Itoh, Y., Takano, K., Hamada, S., Suetsugu, S
  • Journal Title: J .Cell Sci Volume: 124 Pages: 2032-2040
  • DOI: DOI:10.1242/jcs.086264
  • Peer Reviewed
• [Journal Article] NebulinとN-WASPの複合体がIGF-1による筋原線維のアクチン線維形成を担う (2011)
  • Author(s): 高野和儀, 渡邊-高野晴子, 遠藤剛
  • Journal Title: 実験医学 Volume: 29 Pages: 1273-1276
• [Journal Article] (2011)
  • Author(s): 高野和儀, 遠藤剛
  • Journal Title: ライフサイエンス新着論文レビュー
  • URL: http://first.lifesciencedb.jp/archives/1887
• [Journal Article] Nebulin and N-WASP cooperate to causeIGF-1-induced sarcomeric actin filament formation (2010)
  • Author(s): Takano, K., Watanabe-Takano, H., Suetsugu, S., Kurita, S., Tsujita, K., Kimura, S., Karatsu, T., Takenawa, T., Endo, T
  • Journal Title: Science Volume: 330 Pages: 1536-1540
  • DOI: DOI:10.1126/science.1197767
  • Peer Reviewed
• [Presentation] 骨格筋と心筋におけるアクチン重合核形成と伸長の分子機構 (2011)
  • Author(s): 高野和儀, 渡邊-高野晴子, 竹縄忠臣, 遠藤剛
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] 筋収縮と筋再生・筋肥大を担う筋原線維のアクチン線維形成機構 (2011)
  • Author(s): 遠藤剛, 高野和儀
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-21
• [Presentation] NebuletteとN-WASPは心筋筋原線維のアクチン線維形成にかかわっている (2011)
  • Author(s): 高野和儀, 竹縄忠臣, 遠藤剛
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-28
• [Presentation] NebulinとN-WASPは協同して新規のアクチン重合核形成因子としてはたらく (2010)
  • Author(s): 高野和儀, 渡邊-高野晴子, 末次志郎, 竹縄忠臣, 遠藤剛
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同学会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] NebulinとN-WASPはIGF-1による筋原線維アクチンフィラメント形成に関与する (2010)
  • Author(s): 高野和儀, 渡邊-高野晴子, 末次志郎, 竹縄忠臣, 遠藤剛
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-05-19
• [Remarks]
  • URL: http://life.s.chiba-u.jp/endo/hp/index.html
• [Remarks] 研究室ホームページ
  • URL: http://life.s.chiba-u.jp/endo/hp/neb_Nw_newspaper.htm
• [Remarks] 新聞記事