2012 Fiscal Year Final Research Report
Molecular basis of redox signaling that regulates cell motility
| Project/Area Number |
22770189
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Tokyo Institute of Technology (2012)
The University of Tokyo (2010-2011) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 細胞骨格 / 運動 |
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| Research Abstract |
In this project, we have studied how phototaxis of Chlamydomonas is redox-regulated. To clarify the molecular basis of this regulatory pathway, we took three strategies as follows:1) estimation of relationship between state transitions and phototactic signs; 2) identification of responsible genes of three mutants that have defects in redox sensitivity and phototaxis; and 3) identification of proteins that react with thioredoxins that function as dynein subunits. Results are 1) positively phototactic cells are in state 1, whereas negatively phototactic cells are in state 2; 2) one of the mutants has defects in a biosynyhesis pathway of a kind of pigment; and 3) proteins were captured by mutagenized thioredoxins in vivo, although identification of those proteins have not been finished. By further analyses of these results, the molecular mechanism of the regulatory pathway from photoreception to phototaxis will be clarified.
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[Journal Article] The role of retrograde intraflagellar transport in flagellar assembly and function2012
Author(s)
Engel, B.D., Ishikawa, H., Wemmer, K.A.,Geimer, S., Wakabayashi, K., Hirono, M., Craige,B., Pazour, G.J., Witman, G.B., Kamiya, R., andMarshall, W.F.
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Journal Title
Journal of Cell Biology
Volume: 199
Pages: 151-167
DOI
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[Journal Article] bop5 mutations reveal new roles for the IC138 phosphoprotein in the regulation of flagellar behavior2011
Author(s)
VanderWaal, K.E., Yamamoto, R., Wakabayashi, K., Fox, L., Kamiya, R., Dutcher, S.K., Bayly, P., Sale, W.S., and Porter, M.E.
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Journal Title
Molecular Biology of the Cell
Volume: 22
Pages: 2862-74
DOI
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