2011 Fiscal Year Final Research Report
Structural and functional variations of vertebrate cilia
| Project/Area Number |
22770190
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
NARITA Keishi 山梨大学, 大学院・医学工学総合研究部, 講師 (50452131)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 繊毛 / 脈絡 / 上皮細胞 / プロテオミクス / ライブセルイメージング |
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| Research Abstract |
In the present study, we obtained the following results.(1) Proteomic analysis of unique, multiple 9+0 cilia in choroid plexus epithelial cells(CPECs) identified 868 proteins.(2) Live imaging of mouse choroid plexus revealed that neonatal CPEC cilia could beat vigorously, and the motility waned and was lost within 1-2 weeks.(3) Ultrastructural analysis revealed the presence of not only 9+0 but also 9+2 and atypical ciliary subtypes in neonatal CPEC.(4) A set of candidate genes that would regulate ciliary number per cell was identified based on DNA microarray analysis.
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