2011 Fiscal Year Final Research Report
Development of the assay system for chromatin regulatory factors using modified centromere DNA
| Project/Area Number |
22770203
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | かずさDNA研究所 (2011)
Kazusa DNA Research Institute (2010) |
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Principal Investigator |
OHZEKI Junichirou かずさDNA研究所, (ヒトゲノム研究部)細胞工学研究室, 研究員 (30514088)
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| Research Collaborator |
MASUMOTO Hiroshi かずさDNA研究所, (ヒトゲノム研究部)細胞工学研究室, 室長 (70229384)
NAGASE Takahiro かずさDNA研究所, (産業基盤開発研究部)遺伝子応用技術研究室, 室長 (10211442)
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| Project Period (FY) |
2010 – 2011
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| Keywords | セントロメア / キネトコア / ヒト人工染色体 / クロマチン / アルフォイドDNA |
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| Research Abstract |
During the cell proliferation, chromosomes, integral of our DNA information, are needed to be replicated and segregated to each divided daughter cell, correctly. A functional DNA/protein complex named centromere is responsible for chromosome segregation. However, the mechanisms, how centromere structure is organized, is yet unknown. Then, we created the artificial centromere DNA sequence containing a specific protein binding site, and tethered several kinds of chromatin related protein candidates through this binding site. As a result, we found that a kind of protein modification enzyme, histone acetyltransferase, can induce de novo centroemre formation on the centromere DNA sequence.
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