Quantitative live imaging analysis of blood vessel formation in transgenic quail embryo

2011 Fiscal Year Final Research Report

• Project/Area Number: 22770219
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: SATO Yuki 熊本大学, 大学院・先導機構, 特任助教 (90508186)
• Project Period (FY): 2010 – 2011
• Keywords: 発生・分化 / イメージング

Research Abstract

We focused on the unidirectional migration event of dorsal aorta endothelilal cells in early development. We found that such migratory behavior is not autonomous action of individual endothelial cells and got the collateral evidence suggesting existence of some interactions between the dorsal aortae and surrounding tissues. We observed that adjacent somite cells protrude filopodia-like processes during the dorsal aorta migration by time-lapse imaging.

Research Products

• [Journal Article] Dynamic lineage analysis of embryonic morphogenesis using transgenic quail and 4D multispectral imaging (2011)
  • Author(s): Bower, D. V., Sato, Y., and Lansford, R.
  • Journal Title: Genesis Volume: 49 Pages: 619-643
  • Peer Reviewed
• [Journal Article] Dynamic analysis of vascular morphogenesis using transgenic quail embryos (2010)
  • Author(s): Sato, Y., Poynter, G., Huss, D., Filla, M. B., Czirok, A., Rongish, B., Little, C. D., Fraser, S. E., and Lansford, R.
  • Journal Title: PLoS ONE Volume: 5 Pages: e12674
  • Peer Reviewed
• [Presentation] 血管のトランスポジション現象を支える血管内皮細胞の動き (2012)
  • Author(s): 佐藤有紀
  • Organizer: 第117回日本解剖学会総会・全国学術集会(招待講演)
  • Place of Presentation: 山梨大学(山梨)
  • Year and Date: 2012-03-26
• [Presentation] Somitic filopodia are involved in dorsal aorta transposition (2011)
  • Author(s): Sato, Y. and Nagatoshi, K.
  • Organizer: 6th International Chick Meeting
  • Place of Presentation: Roslin institute(Edinburgh, UK)
  • Year and Date: 20110917-20
• [Presentation] トランスジェニックウズラ胚のタイムラプス観察からわかった血管内皮細胞のふるまい (2011)
  • Author(s): 佐藤有紀
  • Organizer: 日本顕微鏡学会第55回シンポジウム(招待講演)
  • Place of Presentation: かがわ国際会議場(香川)
  • Year and Date: 2011-10-01
• [Presentation] Somitic filopodia are required for dorsal aorta transposition (2011)
  • Author(s): Sato, Y. and Nagatoshi, K.
  • Organizer: 44th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄コンベンションセンター(沖縄)
  • Year and Date: 2011-05-20
• [Book] 体節から血管へ、組織の間を旅する細胞 (2012)
  • Author(s): 佐藤有紀
  • Total Pages: 5
  • Publisher: 新曜社
• [Remarks] 熊本大学大学院先導機構研究の紹介
  • URL: http://sendou.kuma-u.jp/research/sato.html
• [Remarks] 研究室ウェブサイト
  • URL: http://bloodvesseldynamics.jp/