2011 Fiscal Year Final Research Report
A transposon suppressor Dart-cancellar in wild rice
| Project/Area Number |
22780007
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
TSUGANE Kazuo 基礎生物学研究所, 多様性生物学研究室, 助教 (50343744)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 植物分子育種 |
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| Research Abstract |
Whole rice genome, which transposon content is 35 %, was sequenced with high accuracy and informative for transposon research. An active rice transposon nDart1-0 element was identified as a causative element for the mutable virescent line that shows green sectors in the virescent yellow leaves. The transposition of nonautonomous nDart1-0 element requires transposase supplied from an autonomous aDart1-27 element. An introduced aDart1-27 element from other variety by crossing has kept the active state. However, a hybrid line obtained by interspecific cross between an African wild rice species, Oryza longistaminata, and a mutable virescent line showed the only green leaf phenotype even though it carried both the nDart1-0 and aDart1-27 elements. Genetic analysis indicated the inhibition of nDart1-0 transposition was caused by a factor derived from Oryza longistaminata. It was revealed that the inhibitory factor regulated transposase mRNA in a posttranscriptional manner
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