2011 Fiscal Year Final Research Report
Analysis of the origin and the mechanism of persistent infection of BmMLV
| Project/Area Number |
22780045
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied entomology
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | BmMLV / マキュラウイルス / 宿主域 / 持続感染 / 培養細胞 / バキュロウイルス発現系 / カイコ / バキュロウイルス |
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| Research Abstract |
To identify the origin of Bombyx mori macula-like virus, we analyzed the host range of BmMLV. Several insect-and mammalian-derived cell lines were inoculated with BmMLV and analyzed for the propagation of BmMLV. We showed that only B. mori-derived cell lines were permissive to BmMLV persistent infection. Also, BmMLV-negative BmVF cells were inoculated with BmNPV. We identified that the recombinant protein was fully expressed and suitable post-translational modification was occurred in BmNPV-infected BmVF cells. These data showed that BmMLV replicates only in B. mori-derived cells and BmVF cell line was suitable for baculovirus expression vector system.
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