Screening and functional analysis of genes responsible for phase-change induction in brown planthopper, Nilaparvata lugens

2012 Fiscal Year Final Research Report

• Project/Area Number: 22780050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: KOBAYASHI Tetsuya 独立行政法人農業生物資源研究所, 昆虫科学研究領域, 主任研究員 (90355321)
• Co-Investigator(Renkei-kenkyūsha): NODA Hiroaki 農業生物資源研究所, 昆虫科学研究領域, 研究専門員 (40343991) ; SUETSUGU Yoshitaka 農業生物資源研究所, 農業生物先端ゲノム研究センター, 主任研究員 (80533471)
• Project Period (FY): 2010 – 2012

Research Abstract

The brown planthopper, Nilaparvata lugens shows two wing forms, macropterous and brachypterous ones. The head and thorax of third instar and fourth instar nymphs were prepared from two different nymphal densities, solitary and crowded conditions. A new micro-array was designed from RNA-seq data obtained from all stages and tissues of N. lugens. The microarray experiments were conducted for screening genes responsible for phase-change of N. lugens.

Research Products

• [Journal Article] A Simple Sequence Repeat- and Single-Nucleotide Polymorphism-Based genetic linkage map of the Brown Planthopper, Nilaparvata lugens (2013)
  • Author(s): Jairin J, Kobayashi T, Yamagata Y, Sanada-Morimura S, Mori K, Tashiro K, Kuhara S, Kuwazaki S, Urio M, Suetsugu Y, Yamamoto K, Matsumura M, Yasui H
  • Journal Title: DNA Res Volume: 20 Pages: 17-30
• [Journal Article] Spider predation on a mirid pest in Japanese rice fields (2011)
  • Author(s): Kobayashi T, Takada M, Takagi S, Yoshioka A, Washitani I
  • Journal Title: Basic Appl Ecol Volume: 12 Pages: 532-539
• [Journal Article] Genetic isolation of the sorghum plant bug Stenotus rubrovittatus (Hemiptera: Miridae) in Fukushima and Ibaraki prefectures (2011)
  • Author(s): Kobayashi T, Matsuki N, Yokosuka T
  • Journal Title: Appl Entomol Zool Volume: 46 Pages: 343-351
• [Journal Article] Multiple origins of outbreak populations of a native insect pest in an agro-ecosystem (2011)
  • Author(s): Kobahashi T, Sakurai T, Sakakibara M, Watanabe T
  • Journal Title: B Entomol Res Volume: 101 Pages: 313-324
• [Presentation] トビイロウンカ近交系を用いた抵抗性イネ品種に対する加害性の遺伝解析 (2013)
  • Author(s): 小林徹也,末次克行,真田幸代,松村正哉
  • Organizer: 第57回日本応用動物昆虫学会大会95
  • Year and Date: 20130000
• [Presentation] イネ吸汁応答性トビイロウンカグルコーストランスポーターの同定 (2013)
  • Author(s): 菊田真吾,小林徹也,黄川田隆洋,中島信彦,野田博明
  • Organizer: 第57回日本応用動物昆虫学会大会205
  • Year and Date: 20130000
• [Presentation] Suetsugu Y, Kobayashi T, Daimon T, Joraku A, Nakamura Y, Kuwazaki S, Kayukawa T, Katayose Y, Kanamori H, Kurita K (2012)
  • Author(s): Suetsugu Y, Kobayashi T, Daimon T, Joraku A, Nakamura Y, Kuwazaki S, Kayukawa T, Katayose Y, Kanamori H, Kurita K
  • Organizer: Nilaparvata lugens XXIV International Congress of Entomology
  • Year and Date: 20120000
• [Presentation] Progress in genome sequencing of the brown planthopper, Nilaparvata lugens (2012)
  • Author(s): Suetsugu Y, Daimon T, Jouraku A, Kobayashi T, Nakamura Y, Kuwazaki S, Kayukawa T, Katayose Y, Kanamori H, Kurita K, Sanada-Morimura S, Matsumura M, Tanaka Y, Tanaka H, Shiotsuki T, Shinoda T, Yamamoto K, Noda H
  • Organizer: 6th Annual Arthropod Genomics Symposium: Arthropod Genomics 2012
  • Place of Presentation: Taking Center Stage and i5k Community Workshop EG-17
  • Year and Date: 20120000
• [Presentation] Strategies to identify genes mediating the virulence of brown plant hopper to resistant rice (2012)
  • Author(s): Kobayashi T, Hattori M
  • Organizer: The 20th Biennial International Plant Resistance to Insects Workshop 23
  • Year and Date: 20120000
• [Presentation] Horizontal webs of Tetragnatha spiders and abundant weeds enhance biological control of insect pests by spiders in organic paddy fields (2012)
  • Author(s): Takada M B, Takagi S, Kobayashi T, Yoshioka A, Kubo T, Washitani I
  • Organizer: Joint Meeting of the 59th Annual Meeting of ESJ & The 5th EAFES International Congress 26
  • Year and Date: 20120000
• [Presentation] 環境保全型水田における広食性捕食者・雑草がイネ害虫に与える形質介在間接効果の重要性 (2011)
  • Author(s): 高田まゆら,高木俊,小林徹也,吉岡明良,鷲谷いつみ
  • Organizer: 第27回個体群生態学会大会33
  • Year and Date: 20110000
• [Presentation] イネウンカ類のバイオタイプ研究の現状と展望 (2011)
  • Author(s): 小林徹也
  • Organizer: 公開シンポジウム「ポストゲノム時代の害虫防除研究のあり方」第4回-ウンカ防除の現状と展望-
  • Year and Date: 20110000
• [Presentation] DNAマーカーを用いた水田のクモ類による害虫の捕食量の推定 (2011)
  • Author(s): 小林徹也,高田まゆら,高木俊,吉岡明良,鷲谷いつみ
  • Organizer: Acta Arachnologica
  • Year and Date: 20110000
• [Presentation] 環境保全型水田におけるクモ類の天敵としての役割 (2011)
  • Author(s): 高田まゆら,小林徹也,高木俊,吉岡明良,鷲谷いつみ
  • Organizer: Acta Arachnologica
  • Year and Date: 20110000
• [Presentation] 遺伝的多様性からみた斑点米カメムシの被害拡大の過程と要因 (2011)
  • Author(s): 小林徹也
  • Organizer: 第27回個体群生態学会大会
  • Year and Date: 20110000