Identification of mechanism of chemoreception for host recognition and feeding habit evolution in a swallowtail butterflies

2012 Fiscal Year Final Research Report

• Project/Area Number: 22780052
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: JT Biohistory Research Hall
• Principal Investigator: OZAKI Katsuhisa 株式会社生命誌研究館, 研究員 (60396223)
• Project Period (FY): 2010 – 2012
• Keywords: アゲハチョウ / 味覚受容体 / 化学感覚 / 生物間相互作用 / 食性進化 / 産卵行動

Research Abstract

Female of swallowtail butterflies select their host plant by tasting using chemo-sensory hair on their tarsi. I identified a mechanism of taste receptor for oviposition behavior. When expression of the receptor gene is interfered by RNAi method, butterflies show reduced sensitivity for the synephrine, one kind of 10 oviposition stimulants. This result suggests that this receptor gene affect to evolution driven by host-shift of phytophagous swallowtail butterflies.

Research Products

• [Journal Article] Gustatory sensing mechanism coding for multiple oviposition stimulants in the swallowtail butterfly (2013)
  • Author(s): Masasuke Ryuda, Delphine Calas-List, Ayumi Yamada, Frederic Marion-Poll, Hiroshi Yoshikawa, Teiichi Tanimura, and Katsuhisa Ozaki
  • Journal Title: Papilio xuthus Journal of Neuroscience Volume: 33 (3) Pages: 914-924
  • DOI: doi: 10.1523/JNEUROSCI.1405-12.2013
  • Peer Reviewed
• [Journal Article] A gustatory receptor involved in host plant recognition for oviposition of a swallowtail butterfly (2011)
  • Author(s): Katsuhisa Ozaki, Masasuke Ryuda, Ayumi Yamada, Ai Utoguchi, Hiroshi Ishimoto, Delphine Calas, Frederic Marion-Poll, Teiichi Tanimura & Hiroshi Yoshikawa
  • Journal Title: Nature Communications
  • DOI: doi:10.1038/ncomms1548
  • Peer Reviewed
• [Presentation] アゲハチョウが産卵のために食草を認識する仕組みに関与する味覚受容体 (2012)
  • Author(s): 尾崎克久・龍田勝輔・吉川寛
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場: 福岡県福岡市(ワークショップ・ポスター)
  • Year and Date: 20121211-14
• [Presentation] アゲハチョウの産卵行動のための食草認識に関与する味覚受容体 (2012)
  • Author(s): 尾崎克久・龍田勝輔・吉川寛
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: (九州大学医学部: 福岡県福岡市)(招待講演)
  • Year and Date: 20120924-26
• [Presentation] A gustatory receptor involved in host-plant recognition for oviposition of the butterfly, Papilio xuthus. (2011)
  • Author(s): Ozaki, Katsuhisa, Ryuda, Masasuke and Yoshikawa, Hiroshi
  • Organizer: The 59th Annual Meeting of the Entomological Society of America.
  • Place of Presentation: アメリカ合衆国ネバダ州リノ市: シンポジウム招待講演
  • Year and Date: 2011-11-16
• [Book] 化学受容の科学第6章 昆虫ゲノムに見る化学感覚受容体遺伝子の多様化 東原和成編 (2012)
  • Author(s): 尾崎克
  • Publisher: 化学同人
• [Book] アゲハチョウが植物を的確に見分けるしくみ. AROMA RESEARCH No.50 2012/5 月号 (2012)
  • Author(s): 尾崎克久・龍田勝輔・吉川寛
• [Remarks] (1) RKB 毎日放送ラジオ局(福岡県)・中西一清スタミナラジオ (2011.12.2)
  • URL: http://www.brh.co.jp/seimeishi/journal/069/research_1.html
• [Remarks] (2) NHK ニュース(関西地区)・おはよう関西 (2011.11.16)
• [Remarks] (1) NHK(久保隆記者)
• [Remarks] (2) 読売新聞(阿部健記者)
• [Remarks] (3) 毎日新聞(須田桃子記者)
• [Remarks] (1) 日本経済新聞(松田記者)
• [Remarks] (2) 共同通信(中沢記者): 写真等の資料を提供
• [Remarks] (3) 朝日新聞(東山記者): 写真等の資料を提供
• [Remarks] (4) 産経新聞(秋山記者)