Live cell imaging of prokaryotic magnetic organelle to analyze mechanisms of organelle formation and function

2012 Fiscal Year Final Research Report

• Project/Area Number: 22780063
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Kanazawa University
• Principal Investigator: TAOKA Azuma 金沢大学, 自然システム学系, 助教 (20401888)
• Project Period (FY): 2010 – 2012
• Keywords: 原核細胞オルガネラ / 生細胞イメージング / 磁気微粒子 / バイオミネラリゼーション / 細菌

Research Abstract

Magnetotactic bacteria possess nano-sized prokaryotic magnetic organelles, magnetosomes. We developed live cell imaging technique by using fluorescence proteins to analyze cellular dynamics of the magnetosomes. By fusions of fluorescence proteins with magnetosomal proteins, we labeled magnetosome membrane and magnetosomal cytoskeleton in Magnetospirillum magneticum AMB-1. We successfully observed segregation of the magnetosomes to daughter cells during cell division.

Research Products

• [Journal Article] Analysis of magnetotactic behavior by swimming assay (2013)
  • Author(s): Sakaguchi, S., Taoka, A., and Fukumori Y
  • Journal Title: Biosci. Biotech. Biochem Volume: (in press)
  • DOI: DOI:10.1271/bbb.120880
  • Peer Reviewed
• [Journal Article] Single-Molecule Imaging on Living Bacterial Cell Surface by High-Speed AFM (2012)
  • Author(s): Yamashita, H, Taoka, A, Uchihashi, T., Asano, T., Ando, T., and Fukumori, Y
  • Journal Title: J. Mol. Biol Volume: 422 Pages: 300-309
  • DOI: DOI:10.1016/j.jmb.2012.05.018
  • Peer Reviewed
• [Journal Article] Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy (2010)
  • Author(s): Yamamoto, D, Taoka, A. Uchihashi,T., Sasaki, H., Watanabe, H., Ando,T., and Fukumori, Y.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Pages: 9382-9387
  • DOI: DOI:10.1073/pnas.1001870107
  • Peer Reviewed
• [Presentation] 生きた細菌細胞表層のナノオーダー構造解析 (2013)
  • Author(s): 田岡 東、福 森 義宏
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2013-03-19
• [Presentation] Functional Analysis of Actin-Like Cytoskeletal Protein MamK Associated with Prokaryotic Organelle Magnetosomes (2013)
  • Author(s): Azuma Taoka, Shoutaro Sakaguchi, and Yoshihiro Fukumori
  • Organizer: The 23rdCDB Meeting: Building multicellular systems form cellular cross-talk
  • Place of Presentation: RIKEN center for developmental biology (兵庫県)
  • Year and Date: 2013-01-22
• [Presentation] 生物磁気微粒子の生合成に関わるヘム蛋白質MamPの機能解析 (2012)
  • Author(s): 江口 友佳子、見世 慎吾、田岡 東、福 森 義宏
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡県)
  • Year and Date: 2012-12-15
• [Presentation] 高速AFMによる生きた原核細胞表面における分子動態イメージング (2012)
  • Author(s): 田岡 東、山下 隼人、内橋 貴之、安藤 敏夫 、福 森 義宏
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡県)
  • Year and Date: 2012-12-15
• [Presentation] Molecular imaging in living magnetotactic bacterial cell surface by high-speed atomic force microscopy (2012)
  • Author(s): Azuma Taoka, Hayato Yamashita, Takayuki Uchihashi, Toshio Ando, Yoshihiro Fukumori
  • Organizer: The 2012 International Meeting on Magnetotactic Bacteria (MTB 2012)
  • Place of Presentation: カリフォルニア大学バークレー校(アメリカ バークレー市)
  • Year and Date: 2012-06-13
• [Presentation] 原核細胞オルガネラ「マグネトソーム」の生細胞イメージング (2012)
  • Author(s): 森井 香,田岡 東, 福森 義宏
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学池袋キャンパス(東京都)
  • Year and Date: 2012-03-11
• [Presentation] 新しい走磁性測定法によるマグネトソーム局在蛋白質の機能解析 (2011)
  • Author(s): 坂口 祥太朗,田岡 東, 福森 義宏
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会議場(京都府)
  • Year and Date: 2011-09-22
• [Presentation] First visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy (2011)
  • Author(s): Yoshihiro Fukumori, Daisuke Yamamoto, Azuma Taoka, Takayuki Uchihashi, Hiroki Watanabe, Toshio Ando
  • Organizer: 第63回日本細胞生物学会年会
  • Place of Presentation: 北海道大学札幌キャンパス(北海道)
  • Year and Date: 2011-06-27
• [Presentation] Structural analysis of nano-sized prokaryotic organelle 'magnetosome ' using atomic force microscopy (2010)
  • Author(s): Azuma Taoka, Daisuke Yamamoto, Takayuki Uchihashi, Hideaki Sasaki, Hiroki Watanabe, Toshio Ando, and Yoshihiro Fukumori
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] New approach for visualization of the ultrastructures of magnetosome using atomic force microscopy (2010)
  • Author(s): Azuma Taoka, Daisuke Yamamoto, Takayuki Uchihashi, Hideaki Sasaki, Hiroki Watanabe, Toshio Ando, and Yoshihiro Fukumori
  • Organizer: International Symposium on Magnetotactic Bacteria and Biomineralization (MTB2010)
  • Place of Presentation: Institute of Geology and Geophysics Chinese Academy of Sciences, (中国)
  • Year and Date: 2010-09-03
• [Remarks]
  • URL: http://pronet.s.kanazawa-u.ac.jp/j/index.html