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2012 Fiscal Year Final Research Report

An Exhaustive Screening and Functional Enhancement of a Useful Genes Related to Hydrogen Production by Using a Genetic Engineering Approach.

Research Project

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Project/Area Number 22780070
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyushu Institute of Technology

Principal Investigator

MAEDA Toshinari  九州工業大学, 大学院・生命体工学研究科, 准教授 (00470592)

Project Period (FY) 2010 – 2012
Keywords水素ガス / バイオテクノロジー / 遺伝子工学 / 生体機能利用 / 代謝工学
Research Abstract

The purpose of this study is to enhance bacterial hydrogen production. During this research activity, an exhaustive screening using an Escherichia coli mutant library was performed to find a useful gene related to hydrogen production, which could be helpful to fully understand biohydrogen production in E. coli; as a result, there are several uncharacterized genes, which have been never reported that they are related to hydrogen production. In addition, the function of E. coli hydrogenases in hydrogen fermentation from glycerol was studied.

  • Research Products

    (18 results)

All 2013 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (8 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Influence of Escherichia colihydrogenases on hydrogen fermentation from glycerol2013

    • Author(s)
      V. Sanchez-Torres, M. Z. Mohd Yusoff, C.Nakano, T. Maeda, H.I. Ogawa, T. K. Wood
    • Journal Title

      International Journal of Hydrogen Energy

      Volume: Vol.38, No.10 Pages: 3905-3912

    • URL

      http://dx.doi.org/10.1016/j.ijhydene.2013.01.031

    • Peer Reviewed
  • [Journal Article] Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production2012

    • Author(s)
      M. Z. Mohd Yusoff, T. Maeda, V. Sanchez-Torres, H. I. Ogawa, Y. Shirai, T. K. Wood
    • Journal Title

      International Journal of Hydrogen Energy

      Volume: Vol.37, No.23 Pages: 17778-17787

    • URL

      http://dx.doi.org/10.1016/j.ijhydene.2012.08.115

    • Peer Reviewed
  • [Journal Article] Bioconversion of glycerol for bioethanol production using isolated Escherichia coli SS12012

    • Author(s)
      S. N. Suhaimi, L.-Y. Phang, T. Maeda,S. Abd-Aziz, M. Wakisaka, Y. Shirai, M. A. Hassan
    • Journal Title

      Brazilian Journal of Microbiology

      Volume: Vol.43, No.2 Pages: 506-516

    • URL

      http://dx.doi.org/10.1590/S1517-83822012000200011

    • Peer Reviewed
  • [Journal Article] Hydrogen production by recombinant Escherichia coli strains2012

    • Author(s)
      T. Maeda, V. Sanchez-Torres, T. K. Wood
    • Journal Title

      Microbial Biotechnology

      Volume: Vol.5, No.2 Pages: 214-225

    • URL

      http://dx.doi.org/10.1111/j.1751-7915.2011.00282.x

    • Peer Reviewed
  • [Journal Article] 高度水素生産菌株を活用した迅速な安全性評価技術の開発2011

    • Author(s)
      前田憲成、 有吉弘貴、 高柳聖、 尾川博昭
    • Journal Title

      用水と廃水

      Volume: 53巻 Pages: 458-464

    • Peer Reviewed
  • [Presentation] Biohydrogen production from sewage sludge and oil palm frond (OPF) juice by a metabolically engineered Escherichia coli strain2013

    • Author(s)
      N. H. Mohd Yasin, T. Maeda, M. Fukuzaki, T. Miyazaki, C. M. H. Che Maail, H.Ariffin
    • Organizer
      平成 24 年度日本水環境学会九州支部大会
    • Place of Presentation
      北九州産業学術推進機構(北九州)
    • Year and Date
      2013-02-16
  • [Presentation] Unrealized function of Escherichia coli genes; sufD and yehP in biohydrogen production2012

    • Author(s)
      M. Z. Mohd Yusoff, T. Maeda, Y.Hashiguchi, T. K. Wood, Y. Shirai, H. I. Ogawa, M. A. Hassan
    • Organizer
      創立 90 周年記念第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2012-10-25
  • [Presentation] グリセロールからの水素発酵における大腸菌ヒドロゲナーゼの機能解析2012

    • Author(s)
      中野智恵理、前田憲成、尾川博昭、V.Sanchez-Torres
    • Organizer
      第46回日本水環境学会年会
    • Place of Presentation
      東洋大学(東京)
    • Year and Date
      2012-03-14
  • [Presentation] 遺伝子工学的組換え微生物による下水余剰汚泥の可溶化成分からの水素発酵2012

    • Author(s)
      福崎雅治、野中智徳、前田憲成、尾川博昭
    • Organizer
      第46回日本水環境学会年会
    • Place of Presentation
      東洋大学(東京)
    • Year and Date
      2012-03-14
  • [Presentation] Functional analysis of an important gene related to biohydrogen production in Escherichia coli, BioMicroWorld 2011, IV International Conference on Environmental2011

    • Author(s)
      M. Z. Mohd Yusoff, T. Maeda, V. SanchezTorres, T. K. Wood, Y. Shirai, H. I. Ogawa, M. A. Hassan
    • Organizer
      Industrial and Applied Microbiology
    • Place of Presentation
      Malaga (Spain)
    • Year and Date
      2011-09-14
  • [Presentation] 高度水素生産菌株を活用した簡易・迅速な毒性検出技術の開発2011

    • Author(s)
      有吉弘貴, 前田憲成, V. Sanchez-Torres, 尾川博昭
    • Organizer
      環境バイオテクノロジー学会 2011 年度大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-06-20
  • [Presentation] 大腸菌ヒドロゲナーゼ変異株によるグリセロールからの水素発酵2011

    • Author(s)
      前田憲成 , 中野智恵理 , V. SanchezTorres, M. Z. Mohd Yusoff, 尾川博昭
    • Organizer
      環境バイオテクノロジー学会 2011 年度大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-06-20
  • [Presentation] Exhaustive Screening of 3985 Isogenic Escherichia coli Mutants for Enhanced Bacterial Hydrogen Production2010

    • Author(s)
      T. Maeda,V. Sanchez-Torres,T. K. Wood
    • Organizer
      2010 American Institute of Chemical Engineers (AIChE) Annual Meeting
    • Place of Presentation
      Salt Lake City (USA)
    • Year and Date
      2010-11-10
  • [Book] 九工大世界トップ技術2011

    • Author(s)
      前田憲成, 中野光一, 尾川博昭
    • Total Pages
      110-122
    • Publisher
      西日本新聞社
  • [Book] Microbial Degradation of Xenobiotics2011

    • Author(s)
      T. Maeda, H. I. Ogawa
    • Total Pages
      213-233
    • Publisher
      Springer
  • [Remarks] (1)大学ホームページにおける研究代表者のデータベース

    • URL

      https://research02.jimu.kyutech.ac.jp/html/333_ja.html

  • [Remarks] (2)研究室のホームページ

    • URL

      http://www.life.kyutech.ac.jp/~toshi.maeda/

  • [Patent(Industrial Property Rights)] 被験物のスクリーニング方法2010

    • Inventor(s)
      前田憲成、尾川博昭
    • Industrial Property Rights Holder
      九州工業大学
    • Industrial Property Number
      特願 2010-056824
    • Filing Date
      2010-03-13

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Published: 2014-08-29  

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