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2011 Fiscal Year Final Research Report

Spx-dependent oxygen sensing mechanism in lactic acid bacteria

Research Project

  • PDF
Project/Area Number 22780074
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKitasato University

Principal Investigator

YAMAMOTO Yuji  北里大学, 獣医学部, 講師 (10453507)

Project Period (FY) 2010 – 2011
Keywords微生物制御 / 発現制御 / 乳酸菌 / 酸素 / 活性酸素 / 連鎖球菌
Research Abstract

In thepresent study, we analyzed Spx-dependent oxygensensing mechanism of Streptococcus mutans. Spx is a RNA-binding protein originally identified in Bacillus subtilis. Both SpxA and SpxB of S. mutanswere shown to more strongly bind to alpha-subunit of RNA polymerase when cysteineresidues of these proteins were oxidized. Analysis of cellular Spx degradation pathway demonstrated that SpxB was degraded by ClpXP protease complexwhilecellular SpxA concentrations were constant and not affected by ClpXP protease. Increased concentrationsof SpxB in clpPor clpXmutant didn't affect the expression profileof antioxidative proteins. Theseresultsindicated that the oxidation of cysteineresidues of Spx, rather than cellularSpx concentrations, might play a critical role in oxygen sensing of S. mutans.

  • Research Products

    (6 results)

All 2011 2010

All Journal Article (1 results) Presentation (2 results) Book (3 results)

  • [Journal Article] プロバイオティクスとして用いられる微生物の低温保存時の挙動と生残性に影響を与える要因2011

    • Author(s)
      山本裕司
    • Journal Title

      日本冷凍空調学会誌冷凍

      Volume: 86(1002) Pages: 317-322

  • [Presentation] 口腔内連鎖球菌における酸素感知機構の解析2011

    • Author(s)
      安部達也、鈴木聡美、山本裕司、向井孝夫
    • Organizer
      日本畜産学会114回大会
    • Place of Presentation
      十和田
    • Year and Date
      2011-08-26
  • [Presentation] 口腔内連鎖球菌の酸素分圧応答に関与するSpx蛋白質の制御機構の解析2011

    • Author(s)
      山本裕司、鈴木聡美、安部達也、向井孝夫
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
  • [Book] Current Progress in Advanced Research2011

    • Author(s)
      Yuji Yamamoto, Philippe Gaudu and Alexandra Gruss, Kenji Sonomoto and Atsushi Yokota
    • Total Pages
      91-102
    • Publisher
      Caister Academic Press
  • [Book] Response of lactic acid bacteria to oxidative stressin Stress Responses of Lactic Acid Bacteria2011

    • Author(s)
      Benedicte Cesselin, Aurelie Derre-Bobillot, Annabelle Fernandez, Gilles Lamberet, Delphine Lechardeur, Yuji Yamamoto, Martin Bastian Pedersen, Christel Garrigues, Alexandra Gruss and Philippe Gaudu
    • Total Pages
      111-128
    • Publisher
      Springer-Verlag Press
  • [Book] 乳酸菌とビフィズス菌のサイエンス、乳酸菌・ビフィズス菌の細胞表層タンパク質と提示機構、乳酸菌における酸素ストレス応答2010

    • Author(s)
      山本裕司
    • Total Pages
      105-112, 213-217
    • Publisher
      京都大学学術出版会

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Published: 2013-07-31  

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