2011 Fiscal Year Final Research Report
Formation of functional complex of enzymes with novel carbon nanotube/peptide composite
| Project/Area Number |
22780090
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
HASHIDA Yasuhiko 京都大学, 物質細胞統合システム拠点, 特定研究員 (30512462)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 酵素 / カーボンナノチューブ / ペプチド / タンパク質工学 / ナノ材料 |
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| Research Abstract |
Complex formation of carbon nanotube(CNT)/peptide composite and enzymes was performed, and it was succeeded that alkaline phosphatase(ALP) was immobilizaed to the CNT/peptide composite as a model enzyme. By the kinetic analysis of enzyme reaction, it was demonstrated that the molecular activity of ALP was retained through the immobilization, while its substrate specificity was reduced. Furthermore, thermal stability of ALP was enhanced by immobilization to CNT/peptide composite, suggesting that it is possible to improve enzyme function via immobilization to CNT/peptide composite.
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