Analysis of the glycosidases on the metabolism ofβ-L-arabinooligosaccharides in Bifidobacteria.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22780094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Kagoshima University
• Principal Investigator: FUJITA Kiyotaka 鹿児島大学, 農学部, 助教 (20381189)
• Project Period (FY): 2010 – 2011
• Keywords: 糖質分解酵素 / 糖鎖 / プレバイオティクス / β-アラビノオリゴ糖鎖 / ビフィズス菌 / 腸内細菌 / エクステンシン / 資化性

Research Abstract

β-L-Arabinooligosaccharides are found in the sugar chains of extensin observed in plant cell wall fractions. In this study, we cloned and characterizedα-L-arabinofuranosidase, novelβ-L-arabinobiosidase and novelβ-L-arabinofuranosidase from Bifidobacterium longum. Furthermore, we revealed a novel degradation pathway forβ-L-arabinooligosaccharides in B. longum.

Research Products

• [Journal Article] Characterization of a novelβ-L-arabinofuranosidase in Bifidobacterium longum : Functional elucidation of a DUF1680 family member (2011)
  • Author(s): Fujita, K., Takashi, Y., Obuchi, E., Kitahara, K., and Suganuma, T.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 38079-38085
  • Peer Reviewed
• [Journal Article] ビフィズス菌由来β-L-アラビノビオシダーゼの機能解析 (2011)
  • Author(s): 藤田清貴、坂元志帆、小野祐樹、若尾雅広、隅田泰生、北原兼文、菅沼俊彦
  • Journal Title: 応用糖質科学 Volume: 1(2) Pages: 153-158
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of aβ-L-arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family (2011)
  • Author(s): Fujita, K., Sakamoto, S., Ono, Y., Wakao, M., Suda, Y., Kitahara, K., and Suganuma, T.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 5143-5150
  • Peer Reviewed
• [Presentation] β-アラビノオリゴ糖鎖のBifidobacterium longumに対する増殖促進効果 (2012)
  • Author(s): 藤田清貴、奥山千代美、北原兼文、菅沼俊彦
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] ビフィズス菌が生産するβ-アラビノオリゴ糖鎖の分解酵素群の解析 (2012)
  • Author(s): 藤田清貴
  • Organizer: 日本農芸化学会西日本支部奨励賞講演会
  • Place of Presentation: 福岡
  • Year and Date: 2012-01-21
• [Presentation] ビフィズス菌によるβ-アラビノオリゴ糖鎖の資化性と関連酵素群の解析 (2011)
  • Author(s): 藤田清貴、大渕衣里子、五反田孝平、北原兼文、菅沼俊彦
  • Organizer: 日本応用糖質科学会平成23年度大会
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-28
• [Presentation] Characterization of novel glycosidases involved in the degradation of Hyp-linked oligoarabinosides (2011)
  • Author(s): Fujita, K., Sakamoto, S., Takashi, Y., Ono, Y., Obuchi, E., Kitahara, K., and Suganuma, T.
  • Organizer: 9th CarbohydrateBioengineering Meeting(CBM9)
  • Place of Presentation: Lisboa, Portugal
  • Year and Date: 2011-05-16
• [Presentation] β-アラビノオリゴ糖鎖の分解に関わる新規糖質分解酵素群の機能解析 (2010)
  • Author(s): 藤田清貴、坂元志帆、高由香里、小野祐樹、大渕衣里子、北原兼文、菅沼俊彦
  • Organizer: 日本生物工学会2010年度大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-29
• [Presentation] ビフィズス菌由来β-L-アラビノビオシダーゼの機能解析 (2010)
  • Author(s): 藤田清貴、坂元志帆、高由香里、小野祐樹、若尾雅広、隅田泰生、北原兼文、菅沼俊彦
  • Organizer: 第17回糖質関連酵素化学シンポジウム
  • Place of Presentation: 静岡
  • Year and Date: 2010-09-17
• [Presentation] ビフィズス菌由来新規β-L-アラビノフラノシダーゼのクローニングと機能解析 (2010)
  • Author(s): 藤田清貴、高由香里、大渕衣里子、北原兼文、菅沼俊彦
  • Organizer: 日本応用糖質科学会平成22年度大会
  • Place of Presentation: 静岡
  • Year and Date: 2010-09-15
• [Remarks]
  • URL: http://hypba2.jimdo.com/