2012 Fiscal Year Final Research Report
Study of enzymes and factors provide direct solubilization of chitin
| Project/Area Number |
22780097
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Ichinoseki National College of Technology |
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Principal Investigator |
NAKAGAWA Yuko 一関工業高等専門学校, 物質化学工学科, 助教 (70435577)
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| Project Period (FY) |
2010 – 2012
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| Keywords | キチン / キチン分解酵素 / 糖化促進 / キチン結合タンパク / N-アセチルグルコサミン / 組換え酵素 |
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| Research Abstract |
We revealed three chitin binding proteins (CBPs) from Streptomyces griseus boosted enzymatic solubilization of - and -chitin. The amount of (GlcNAc)2 from the best combination with one of the CBP and a chitinase was about 30 times higher than that without the CBP after 24 h incubation.We proved crystallinity was affected to -chitin solubilization by using a commercially available enzyme from S. griceus or the chitinolytic system in Serratia marcescens.
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Research Products
(21 results)
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[Journal Article] Development of innovative technologies to decrease the environmental burdens associated with using chitin as a biomass resource:mechanochemical grinding and enzymatic degradation.2011
Author(s)
Yuko S. Nakagawa, Yasuhiro Oyama, Nobuko Kon, Mitsuru Nikaido, Koichi Tanno, Jun Kogawa, Shoji Inomata, Ayano Masui, Akihiro Yamamura, Mitsuaki Kawaguchi, Yoshiharu Matahira, Kazuhide Totani
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Journal Title
Carbohydrate polymers
Volume: 83
Pages: 1843-1849
Peer Reviewed
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[Presentation] Advanced utilization of chitin resources with technology for reducing environmental loads;2010
Author(s)
Kazuhide TOTANI, Yuko NAKAGAWA,Nobuko KON, Yasuhiro OYAMA, Mitsuru NIKAIDO, Mitsumasa OSADA, Mikio KAIHARA, Koichi TANNO, Jun KOGAWA,Shoji INOMATA, Ayano MASUI, Akihiro YAMAMURA, Mitsuaki KAWAGUCHI and Yoshiharu MATAHIRA
Organizer
Mechanochemical treatment and mechanism of enzymatic degradation. 25th International Carbohydrate Symposium
Place of Presentation
幕張
Year and Date
20100801-06
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[Presentation] Pretreatments and enzymatic degradation of chitin.2010
Author(s)
Kazuhide Totani, Yuko Nakagawa, Mitsumasa Osada, Mikio Kaihara, Mitsuru Nikaido, Jun Kogawa, Shoji Inomata, Mitsuaki Kawaguchi,Ayano Masui, Akihiro Yamamura, Yoshiharu Matahira
Organizer
Plant Polysaccharide and Applied Glycoscience
Year and Date
20100729-31
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