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2012 Fiscal Year Final Research Report

Characterization and structural analysis of novel dye-linked L-proline dehydrogenase

Research Project

  • PDF
Project/Area Number 22780098
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionUniversity of Fukui (2011-2012)
Yonago National College of Technology (2010)

Principal Investigator

SATOMURA Takenori  福井大学, 大学院・工学研究科, 准教授 (50412317)

Research Collaborator SAKURABA Haruhiko  香川大学, 農学部, 教授 (90205823)
OHSHIMA Toshihisa  九州大学, 農学研究科, 教授 (10093345)
Project Period (FY) 2010 – 2012
Keywords超好熱菌 / 色素依存性脱水素酵素 / L-プロリン脱水素酵素
Research Abstract

Until now, two different types of Dye-ProDHs have been discovered in hyperthermophile. We have found the third novel type of Dye-ProDH whose molecular composition and properties were completelydifferent. Structure determination was performed. As the result, we succeeded the structural determination at high resolution. In addition, we succeeded the identification of the amino acid which is involved in substrate affinity and electron-transfer of the enzyme.

  • Research Products

    (10 results)

All 2012 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (5 results)

  • [Journal Article] フラビン含有色素依存性脱水素酵素の機能・構造解析とその応用2012

    • Author(s)
      里村武範、川上竜巳、櫻庭春彦、大島敏久
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 70巻 Pages: 340-345

  • [Journal Article] Crystal structure of novel dye-linked L-proline dehydrogenase from hyperthermophilic archaeon Aeropyrum pernix.2012

    • Author(s)
      H. Sakuraba, T. Satomura, R. Kawakami, K. Kim, Y. Hara, K. Yaneda, T. Ohshima
    • Journal Title

      J. Biol. Chem.

      Volume: 287 Pages: 20070-20080

    • Peer Reviewed
  • [Journal Article] Gene expression and characterization of a third type of dye-linked L-proline dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix.2012

    • Author(s)
      T. Satomura, Y. Hara, S. Suye, H. Sakuraba, T. Ohshima
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 76 Pages: 589-593

    • Peer Reviewed
  • [Journal Article] Characterization of a novel dye-linked L -proline dehydrogenase from an aerobic hyperthermophilic archaeon,Pyrobaculum calidifontis.2011

    • Author(s)
      T. Satomura, X. Zhang, Y. Hara, K. Doi,H. Sakuraba, T. Ohshima
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: 89 Pages: 1075-1082

    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysisof a novel dye-linked L-proline dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix2010

    • Author(s)
      T. Satomura, H. Sakuraba, Y. Hara, T. Ohshima
    • Journal Title

      Acta. Crystallogr. Sect. F Struct. Biol. Cryst. Commun.

      Volume: 66 Pages: 1508-1510

    • Peer Reviewed
  • [Presentation] Functional and structural characterization of Dye-linked L-proline dehydrogenase from hyperthermophiles2012

    • Author(s)
      T. Satomura, R. Kawakami, S. Suye, H. Sakuraba, T. Ohshima
    • Organizer
      9th International Congress On Extremophiles
    • Place of Presentation
      Hotel NH Central Convencionesセビリア(スペイン)
    • Year and Date
      2012-09-13
  • [Presentation] 好気性超好熱アーキアAeropyrum pernix由来色素依存性L-プロリン脱水素酵素の構造解析2012

    • Author(s)
      里村武範、川上竜巳、末信一朗、櫻庭春彦、大島敏久
    • Organizer
      2012年度農芸化学会大会
    • Place of Presentation
      京都女子大学、京都
    • Year and Date
      2012-03-23
  • [Presentation] クレンアーキオータに存在する新規ホモダイマー型色素依存性L-プロリン脱水素酵素の機能解析2011

    • Author(s)
      里村武範、川上竜巳、末信一朗、櫻庭春彦、大島敏久
    • Organizer
      第12回極限環境生物学会
    • Place of Presentation
      長崎大学、長崎県
    • Year and Date
      2011-11-28
  • [Presentation] 色素依存性脱水素酵素の網羅的な機能解析2010

    • Author(s)
      里村武範、櫻庭春彦、大島敏久
    • Organizer
      日本農芸化学会中四国支部例会第28回講演会
    • Place of Presentation
      香川大学、香川
    • Year and Date
      2010-09-25
  • [Presentation] クレンアーキオータに存在する新規色素依存性L-プロリン脱水素酵素の構造と機能解析2010

    • Author(s)
      里村武範、原佑介、櫻庭春彦、大島敏久
    • Organizer
      酵素補酵素研究会
    • Place of Presentation
      西日本総合展示場、福岡
    • Year and Date
      2010-09-11

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Published: 2014-08-29  

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