2011 Fiscal Year Final Research Report
Functional design of influenza virus blocker utilizing glycans
| Project/Area Number |
22780101
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Shizuoka University |
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Principal Investigator |
OGATA Makoto 静岡大学, 創造科学技術大学院, 特任助教 (10532666)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 分子認識 |
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| Research Abstract |
Artificial glycoclusters with various backbones(γ-polyglutamic acid, glycopolymer, and EGTA etc…) and multivalent sialyloligosaccharide units have been chemoenzymatically synthesized as potential polymeric inhibitors of infection by influenza virus. Furthermore, in order to synthesize an artificial sialoglycoclusters, we developed a large-scale production of ratα2, 3-orα2, 6-sialyltransferases(α2, 3-orα2, 6-SiaTs). Theα2, 3-orα2, 6-SiaTs were expressed in fifth instar silkworm larval hemolymph using recombinant both cysteine protease-and chitinase-deficient Bombyx mori nucleopolyhedrovirus(Bm NPV-CP-Chi-) bacmid. The expressedα2, 3-SiaT andα2, 6-SiaT were used for sialylation of asialoglycoclusters.
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[Journal Article] Design and synthesis of high-avidity tetravalent glycoclusters as probes for Sambucus sieboldiana agglutinin and characterization of their binding properties.2012
Author(s)
Ogata, M., Yano, M., Umemura, S., Murata, T., Park, E. Y., Kobayashi, Y., Asai, T., Oku, N., Nakamura, N., Matsuo, I., Usui, T.
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Journal Title
Bioconjugate Chem.
Volume: 23
Pages: 97-105
Peer Reviewed
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[Journal Article] Label-free detection of oligosaccharide-lectin interaction using plasmonic optical device for glycomics application.2011
Author(s)
Endo, T., Matsuda, S., Obara, T., Chuma, Y., Ogata, M., Yanagida, Y., Hatsuzawa, T., Usui, T.
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Journal Title
Sensor. Mater.
Volume: 23
Pages: 135-146
Peer Reviewed
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[Journal Article] Lacto sylamidine-based affinity purification for cellulolytic enzymes EG I and CBH I form Hypocrea jecorina and their properties.2010
Author(s)
Ogata, M., Kameshima, Y., Hattori, T., Michishita, K., Suzuki, T., Kawagishi, H., Totani, K., Hiratake, J., Usui, T.
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Journal Title
Carbohydr. Res.
Volume: 345
Pages: 2623-2629
Peer Reviewed
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[Journal Article] Molecular design of fluorescent labeled glycosides as acceptor substrates for sialyl transferases.2010
Author(s)
Ogata, M., Obara, T., Chuma, Y., Murata, T., Park, E. Y., Usui, T.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 74
Pages: 2287-2292
Peer Reviewed
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[Journal Article] Molecular design of N-linked tetravalent glycosides bearing N-acetylglucosamine, N, N'-diacetylchitobiose and N-acetyl lactosamine : Analysis of cross-linking activities with WGA and ECA lectins.2010
Author(s)
Masaka, R., Ogata, M., Misawa, Y., Yano, M., Hashimoto, C., Murata, T., Kawagishi, H., Usui, T.
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Journal Title
Bioorg. Med. Chem.
Volume: 18
Pages: 621-629
Peer Reviewed
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[Presentation] Lactosy lamidine-based affinity purification for cellulolytic enzymes EG I and CBH I and analysis of their condensation and transglycosylation reactions2010
Author(s)
Ogata, M., Kameshima, Y., Hattori, T., Totani, K., Nakamura, T., Koshino, H., Hiratake, J., Usui, T
Organizer
Plant polysaccharide and applied glyco science workshop(PPAGW)
Place of Presentation
The Sasakawa Hall(東京都)
Year and Date
20100729-31
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