2012 Fiscal Year Final Research Report
Evaluation of the in vivo activity of phase II drug-metabolizing enzymes using soy isoflavone metabolism
| Project/Area Number |
22780125
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Food science
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| Research Institution | Kyorin University |
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Principal Investigator |
HOSODA Kaori 杏林大学, 保健学部, 助教 (60433728)
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| Research Collaborator |
ISHII Kazuo 杏林大学, 保健学部, 教授 (80137968)
KATO Tomohisa 杏林大学, 保健学部, 准教授 (80152726)
FURUTA Takashi 東京薬科大学, 薬学部, 教授 (70120152)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 大豆イソフラボン / 薬物代謝酵素 / UGT / SULT |
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| Research Abstract |
In this study, we investigated the activity of drug-metabolizing enzymes such as UGT and SULT using soy isoflavone metabolism in vivo. The results of enzymatic study indicated that UGT1A9 mainly catalyzed 7-glucuronidation of genistein. The quantification of 16 isoflavone metabolites in human plasma revealed that daidzein-7-glucuronide-4'-sulfate and genistein-7-glucuronide-4'-sulfate were major metabolites. Further study about drug-metabolizing enzymes catalyzing the glucuronidation and sulfation of isoflavones is being performed.
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