Identification and assessment of probiotic factor(s) via multiple omics approach.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22780132
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: FUKUDA Shinji 独立行政法人理化学研究所, 免疫系構築研究チーム, 研究員 (80435677)
• Project Period (FY): 2010 – 2011
• Keywords: ビフィズス菌 / 酢酸 / O157 / マルチオーミクス / プロバイオティクス / 腸内細菌 / 相互作用 / 病原性大腸菌

Research Abstract

In order to demonstrate the effect of the bifidobacterial genes encoding 'probiotic transpoter', we generated the genes deficient or overexpressing bifidobacterial strains and assessed them using Escherichia coli O157 : H7 lethal infection murine model. Our experiments clearly showed that the 'probiotic transpoter' largely contributes to the production of acetate by colonized bifidobacteria in the murine colon, and it could prevent the inflammation provoked by Escherichia coli O157 : H7 infection.

Research Products

• [Journal Article] Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters (2012)
  • Author(s): Fukuda, S., Toh, H., Taylor, T. D., Ohno, H., and Hattori, M
  • Journal Title: Gut Microbes Volume: (in press)
  • Peer Reviewed
• [Journal Article] Bifidobacteria can protect from enteropathogenic infection through production of acetate (2011)
  • Author(s): Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., Tobe, T., Clarke, J. M., Topping, D. L., Suzuki, T., Taylor, T. D., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., and Ohno, H
  • Journal Title: Nature Volume: 469 Pages: 543-547
  • Peer Reviewed
• [Journal Article] A dynamic omics approach identifies nutrition-mediated microbial interactions (2011)
  • Author(s): Nakanishi, Y., Fukuda, S., Chikayama, E., Kimura, Y., Ohno, H., and Kikuchi, J
  • Journal Title: J. Proteome Res Volume: 10 Pages: 824-836
  • Peer Reviewed
• [Journal Article] Application of a mouse ligated Peyer's patch intestinal loop assay to evaluate bacterial uptake by M cells (2011)
  • Author(s): Fukuda, S., Hase, K., and Ohno, H
  • Journal Title: J. Vis. Exp Volume: 58 Pages: e3225
  • Peer Reviewed
• [Journal Article] The epithelia-specific membrane trafficking factor AP-1B secures gut immune homeostasis in mice (2011)
  • Author(s): Takahashi, D., Hase, K., Kimura, S., Nakatsu, F., Ohmae, M., Mandai, Y., Sato, T., Date, Y., Ebisawa, M., Kato, T., Obata, Y., Fukuda, S., Kawamura, Y., Dohi, T., Katsuno, T., Yokosuka, O., Waguri, S., and Ohno, H.
  • Journal Title: Gastroenterology Volume: 141 Pages: 621-632
  • Peer Reviewed
• [Journal Article] Complete genome sequences of rat and mouse segmented filamentous bacteria, a potent inducer of Th17 cell differentiation (2011)
  • Author(s): Prakash, T., Oshima, K., Morita, H., Fukuda, S., Imaoka, A., Kumar, N., Sharma, V. K., Takahashi, M., Saitou, N., Taylor, T. D., Ohno, H., Umesaki, Y., and Hattori, M.
  • Journal Title: Cell Host Microbe Volume: 10 Pages: 273-284
  • Peer Reviewed
• [Journal Article] Bifidobacterium kashiwanohaense sp. nov., isolated from healthy infant faeces (2011)
  • Author(s): Morita, H., Nakano, A., Onoda, H., Toh, H., Oshima, K., Takami, H., Murakami, M., Fukuda, S., Takizawa, T., Kuwahara, T., Ohno, H., Tanabe S., and Hattori, M.
  • Journal Title: Int. J. Syst. Evol.Microbiol Volume: 61 Pages: 2610-2615
  • Peer Reviewed
• [Journal Article] ビフィズス菌が産生する酢酸が病原性大腸菌感染を予防する:糖トランスポーターの重要性 (2011)
  • Author(s): 福田真嗣
  • Journal Title: 科研費NEWS Volume: 1 Pages: 17
• [Journal Article] 食品と腸内細菌叢 (2011)
  • Author(s): 福田真嗣、大野博司
  • Journal Title: 臨床検査 Volume: 55 Pages: 183-187
• [Journal Article] ビフィズス菌の産生する酢酸が病原性大腸菌感染を予防する-糖トランスポーターの重要性- (2011)
  • Author(s): 福田真嗣、服部正平、大野博司
  • Journal Title: 実験医学 Volume: 29 Pages: 923-926
• [Journal Article] Development of a monitoring approach for metabolic dynamics in microbial ecosystems by stable isotope labeling technologies (2010)
  • Author(s): Date, Y., Nakanishi, Y., Fukuda, S., Kato, T., Tsuneda, S., Ohno, H., and Kikuchi, J.
  • Journal Title: J. Biosci. Bioeng Volume: 110 Pages: 87-93
  • Peer Reviewed
• [Journal Article] 腸管出血性大腸菌O157H7投与マウスモデルを用いた宿主-腸内フローラ間相互作用の解析 (2010)
  • Author(s): 福田真嗣、大野博司
  • Journal Title: 日本臨床腸内微生物学会誌 Volume: 12 Pages: 35-40
• [Presentation] Integrated omics approach identified acetate produced by probiotic bifidobacteria to protect host from enteropathogenic infection (2012)
  • Author(s): Fukuda, S
  • Organizer: The 4th Stage Surface Barrier Immunology Study Group(SBARIS) 3rd Meeting
  • Place of Presentation: Osaka
  • Year and Date: 20120300
• [Presentation] Bifidobacteria can protect from enteropathogenic infection through production of acetate (2012)
  • Author(s): Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., Tobe, T., Clark, JM., Topping, DL., Suzuki, T., Taylor, TD., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., Ohno, H.
  • Organizer: Keystone Symposia
  • Place of Presentation: The Microbiome, Keystone, Colorado
  • Year and Date: 2012-03-05
• [Presentation] Acetate producing bifidobacteria equipped with 'probiotic transporters' protects the host against enteropathogenic infection (2011)
  • Author(s): Fukuda, S. and Ohno, H
  • Organizer: International symposium, the 40^<th> annual meeting of the Japanese society for Immunology
  • Place of Presentation: Chiba
  • Year and Date: 2011-11-29
• [Presentation] Application of statistical correlation method for understanding complex gut ecosystem (2011)
  • Author(s): Fukuda, S., Nakanishi, Y., Date, Y., Ohno, H., Kikuchi, J
  • Organizer: The International Symposium on Nuclear Magnetic Resonance 2011(ISNMR 2011)
  • Place of Presentation: Yokohama
  • Year and Date: 2011-11-12
• [Presentation] Bifidobacteria can protect from enteropathogenic infection through production of acetate (2011)
  • Author(s): Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., Tobe, T., Clark, JM., Topping, DL., Suzuki, T., Taylor, TD., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., Ohno, H
  • Organizer: Keystone Symposia : Malnutrition, Gut-Microbial Interactions and Mucosal Immunity to Vaccines
  • Place of Presentation: New Delhi
  • Year and Date: 2011-11-09
• [Presentation] マルチオーミクス解析から見えてきた腸内エコシステムの様相 (2011)
  • Author(s): 福田真嗣
  • Organizer: 第6回メタボロームシンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-13
• [Presentation] マルチオーミクス解析技術が解き明かす腸内エコシステムの様相 (2011)
  • Author(s): 福田真嗣
  • Organizer: 2011年度乳酸菌・腸内細菌工学研究部会講演会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-28
• [Presentation] Integrated omics approach identified acetate produced by probiotic bifidobacteria to protect host from enteropathogenic infection (2011)
  • Author(s): Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., Tobe, T., Clark, JM., Topping, DL., Suzuki, T., Taylor, TD., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., Ohno, H
  • Organizer: 15th International Congress of Mucosal Immunology
  • Place of Presentation: Paris
  • Year and Date: 2011-07-07
• [Presentation] Carbohydrate transporters confer a probiotic effect to bifidobacteria (2011)
  • Author(s): Fukuda, S
  • Organizer: The International Scientific Conference on Probiotics and Prebiotics 2011
  • Place of Presentation: Kosice, Slovakia
  • Year and Date: 2011-06-14
• [Presentation] 腸管出血性大腸菌O157 : H7感染死マウスモデルを用いたビフィズス菌による感染死抑止機構の解明 (2011)
  • Author(s): 福田真嗣、藤英博、長谷耕二、大島健志朗、中西裕美子、吉村和敏、戸邉亨、鈴木徹、伊藤喜久治、菊地淳、森田英利、服部正平、大野博司
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-27
• [Presentation] Multiple omics identified probiotic bacterial metabolites to prevent gut inflammation provoked by enterohemorrhagic Escherichia coli O157 : H7 infection (2010)
  • Author(s): Fukuda、S., Toh, H., Nakanishi, Y., Hase, K., Oshima, K., Yoshimura, K., Tobe, T., Taylor, T. D., Itoh, K., Kikuchi, J., Morita, H., Hattori, M., Ohno, H
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-26
• [Book] 微生物機能学第5章代謝経路を人工的に修飾して物質を効率よく生産する-代謝制御発酵- (2012)
  • Author(s): 福田真嗣
  • Total Pages: 62-70
  • Publisher: 三共出版
• [Book] 世紀を超えるビフィズス菌の研究～その基礎と臨床応用から製品開発へ～第2章2部ビフィズス菌定着マウスのマルチオーミクス解析 (2011)
  • Author(s): 福田真嗣
  • Total Pages: 165-170
  • Publisher: 財団法人日本ビフィズス菌センター
• [Book] 難培養微生物研究の最新技術II～ゲノム解析を中心とした最前線と将来展望～第14章微生物群集のメタボローム統合化解析 (2010)
  • Author(s): 福田真嗣
  • Total Pages: 147-155
  • Publisher: シーエムシー出版
• [Remarks] 福田真嗣、大野博司ビフィズス菌が産生する酢酸による病原性大腸菌感染症の予防、大学共同利用機関法人情報・システム研究機構ライフサイエンス統合データベースセンターライフサイエンス新着論文レビュー(2011)
  • URL: http://first.lifesciencedb.jp/archives/2284#more-2284