2012 Fiscal Year Final Research Report
The role of MDR in the elimination of xenobiotics in farmed fish
| Project/Area Number |
22780174
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
KUNIHIKO Futami 東京海洋大学, 海洋科学技術研究科, 助教 (00513459)
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| Project Period (FY) |
2010 – 2012
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| Keywords | MDR / PXR / 薬物代謝 / 肝臓 / エンドスルファン / 農薬 / ティラピア |
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| Research Abstract |
In mammals, drug transporters such as MDR play important roles for elimination of various xenobiotics across extra-cellular membranes. However, the knowledge about the gene expression and function of MDR in farmed fish are relatively modest. Here, we examined the expression of MDR protein in liver of Nile tilapia Oreochromis niloticus treated with the endosulfan, an organochloride pesticide. The protein level of tilapia MDR was increased in a time-dependent manner by feeding with endosulfan. However, there was poor correlation between MDR level and endosulfan residues. We also isolated the full-length cDNAs of MDR and its regulator, PXR from tilapia. A lot of polymorphisms and species difference were found in both MDR and PXR gene. Phylogenetic analysis indicated that, unlike mammal, fish MDR has not differentiated into MDR1 and MDR3. Although tilapia PXR and P-gp mRNAs were detected in various tissues, the differential expression patterns were observed by real-time PCR. These results may imply that the transcription of tilapia MDR is regulated by different mechanism from that of mammalian MDR1/3.
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[Remarks] Oreochromis niloticus P-gp mRNA for P-glycoprotein, complete cds, DDBJ accession no.: AB699096
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[Remarks] Oreochromis niloticus pxr mRNA for pregnane X receptor, complete cds, DDBJ accession no.: AB699095
