Investigation of Brachionus plicatilis species complex classification based on full mitochondrial genome

2011 Fiscal Year Final Research Report

• Project/Area Number: 22780178
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Nagasaki University
• Principal Investigator: SUGA Koushirou 長崎大学, 大学院水産・環境科学総合研究科, 准教授 (60569185)
• Project Period (FY): 2010 – 2011
• Keywords: シオミズツボワムシ / ミトコンドリアDNA / 分類法 / PCR

Research Abstract

RFLP analysis using mtDNA was performed to establish a new classification method matched with biological characters on Brachionus plicatilis species complex. RFLP finger print patterns of mtDNA were inconsistent with its biological characters. Mate Recognition Pheromone gene sequences similarity analysis on B. plicatilis was nearly matched with its biological character classification.

Research Products

• [Journal Article] Axenic culture of Brachionus plicatilis using antibiotics (2011)
  • Author(s): Suga, K., Tanaka, Y., Sakakura, Y., Hagiwara, A.
  • Journal Title: Hydobiologia Volume: 662 Pages: 113-119
  • Peer Reviewed
• [Journal Article] Isolation of mixis related genes from the rotifer Brachionus plicatilis using subtractive hybridization method (2011)
  • Author(s): Suga, K., Oshiyama, N., Tanaka, Y., Sakakura, Y., Hagiwara, A.
  • Journal Title: Hydobiologia Volume: 662 Pages: 83-86
  • Peer Reviewed
• [Presentation] ワムシの生活史に与える光波長の影響 (2011)
  • Author(s): 金禧珍、萩原篤志、菅向志郎
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 20110300
• [Presentation] セレン強化クロレラの給餌がシオミズツボワムシの増殖に与える影響 (2010)
  • Author(s): 中村航、阪倉良孝、菅向志郎、丸山功、萩原篤志
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学
  • Year and Date: 20101000
• [Book] Chapter 7, A role for molecular studies in unveiling the pathways for formation of rotifer resting eggs and their survival during dormancy. Lubzens, E., Cerd` a J. and M. Clark(eds). Dormancy and Resistance in Harsh Environments (2010)
  • Author(s): Denekamp, N. Y., Suga, K., Hagiwara, A., Reinhardt, R., Lubzens, E.
  • Total Pages: 109-132
  • Publisher: Topics in Current Genetics 21