2011 Fiscal Year Final Research Report
Project/Area Number |
22780192
|
Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Fisheries chemistry
|
Research Institution | Tokyo University of Marine Science and Technology |
Principal Investigator |
HAGA Yutaka 東京海洋大学, 海洋科学部, 准教授 (00432063)
|
Project Period (FY) |
2010 – 2011
|
Keywords | 水族栄養 / ブリ / 骨格異常 / モデル / ビタミン |
Research Abstract |
Fluorescent calcein staining successfully visualized cleithrum, and dentary, gill arch and neurocranium of yellowtail at 2 and 14 days post hatching(dph), respectively. Calcein staining also visualized neural spine and other elements of back bone around 15 dph. However, pigment formation at the surface of fish body hinder direct observation of back bone of fish at around 18 dph. Moreover, thickening of body often prevented direct observation of axial skeleton in fish at around 22 dph and becomes impossible at 27 dph. To analyze coincidence of growth depression and occurrence of skeletal deformity, occurrence of bone deformity was examined in fish showing different sizes from same batch of fish. in the 1^<st> experiment, significantly higher incidence of skeletal defomrity was observed in fish from bigger size of fish. However, in apparent contrat, smaller size fish showed higher frequency of occurrence of skeletal deformity. Therefore, there was no clear relationship between growth rate and occurrence of skeletal deformity in fish.
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