Molecular mechanism of environmental stress-induced apoptosis in fish

2012 Fiscal Year Final Research Report

• Project/Area Number: 22780193
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Fisheries chemistry
• Research Institution: Nihon University
• Principal Investigator: YABU Takeshi 日本大学, 生物資源科学部, ポストドクトラルフェロー (00551756)
• Project Period (FY): 2010 – 2012
• Keywords: 脂質生化学 / 酵素 / ストレス / アポトーシス

Research Abstract

The neutral sphingomyelinase １ (nSMase1) is involved in environmental stress-i duced apoptosis infish. To reveal the molecular mechanism, we examined the invo vement of the stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) system. Upon heat shock treatment, the protein kinase initiated a signal transduction pathway leading to cell death (apoptosis). Using recombinant JNK1 protein, we found that phosphorylation of nSMase1 occurred at serine-270. Treatment with heat shock rapidly enhanced the nSMase1-directed kinase activity of endogenous JNK. On the other hand, the mutant of JNK1 dominant negative was much less sensitve to heat shock. Our results strongly suggest that phosphorylation of nSMase1 in heat-shock-induced apoptosis is executed by JNK.

Research Products

• [Journal Article] Molecular cloning of two gonadotropin receptors in mummichog Fundulus heteroclitus and their gene expression during follicular development and maturation. (2013)
  • Author(s): M. Ohkubo, T .Yabu,M. Yamashita, A. Shimizu.
  • Journal Title: Gen.Comp.Endocrinol. Volume: 184 Pages: 75-86
  • Peer Reviewed
• [Journal Article] Induction of autophagy by amino acid starvation in fish cells. (2012)
  • Author(s): T. Yabu, S. Imamura, N. Mizusawa, K. Touhata, M. Yamashita.
  • Journal Title: Mar.Biotechnol. Volume: 14(4) Pages: 491-501
  • Peer Reviewed
• [Journal Article] Protective Role of Cdc48 against Neurodegeneration via Ubiquitin-Proteasome System Dysfunction during Zebrafish Development. (2012)
  • Author(s): S. Imamura, T. Yabu, M. Yamashita.
  • Journal Title: J.Biol.Chem. Volume: 287(27) Pages: 23047-23056
  • Peer Reviewed
• [Journal Article] Purification and characterization of glutathione peroxidase 1 in the red muscle of Pacific bluefin tuna Thunnusorientalis. (2012)
  • Author(s): Y. Yamashita, T. Yabu, K. Touhata, M. Yamashita.
  • Journal Title: Fish.Sci. Volume: 78 Pages: 407-413
  • Peer Reviewed
• [Journal Article] Antiviral protection mechanism mediated by Ginbuna crucian carp interferon gamma isoforms 1 and 2 throtwo distinct interferon-gamma-receptors. (2011)
  • Author(s): T. Yabu, H. Toda, Y. Shibasaki, M. Yamashita, H. Anzai, N. Mano, Y. Masuhiro, S. Hanazawa, H. Shiba, T. Moritomo, T. Nakanishi.
  • Journal Title: J.Biochem. Volume: 150(6) Pages: 635-648
  • Peer Reviewed
• [Journal Article] Selenoneine, total selenium and total mercury content infish muscle. (2011)
  • Author(s): Y. Yamashita, H. Amlund, T. Suzuki, T. Hara, M. A. Hossain, T.Yabu, K. Touhata, M. Yamashita.
  • Journal Title: Fish. Sci Volume: 77(4) Pages: 679-689
  • Peer Reviewed
• [Journal Article] Evaluating antigen-specific cytotoxicity of CD8^+ T cells in fish by granzyme B-like activity. (2011)
  • Author(s): H. Toda, T. Yabu, H. Shiba, T. Moritomo, T. Nakanishi.
  • Journal Title: Vet. Immunol .Immunopathol Volume: 141(1-2) Pages: 168-172
• [Journal Article] Differential gene expression of HSC70/HSP70 in yellowtail cells in response to chaperone-mediated autophagy. (2011)
  • Author(s): T. Yabu, S. Imamura, M. S. Mohammed, K. Touhata, T. Minami, M. Terayama, M. Yamashita.
  • Journal Title: FEBS J. Volume: 278(4) Pages: 673-685
  • Peer Reviewed
• [Journal Article] Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish. (2011)
  • Author(s): H. Toda, Y. Saito, T. Koike, F. Takizawa,K. Araki, T. Yabu, T. Somamoto, H. Suetake, Y. Suzuki, M. Ototake, T. Moritomo, T. Nakanishi.
  • Journal Title: Dev .Comp.Immunol. Volume: 35(6) Pages: 650-660
  • Peer Reviewed
• [Journal Article] Clonal growth of carp (Cyprinus carpio) T cells in vitro. (2011)
  • Author(s): T. Yamaguchi, F. Katakura, S. Shitanda, Y. Niida, H. Toda, M. Ohtani, T . Yabu, H. Suetake, T. Moritomo, T. Nakanishi.
  • Journal Title: Dev. Comp. Immunol. Volume: 35(2) Pages: 193-202
  • Peer Reviewed
• [Journal Article] Stress protein HSP70 in fish. (2010)
  • Author(s): M. Yamashita, T. Yabu, N. Ojima.
  • Journal Title: Aqua-BioSci. Monogr. Volume: 3(4) Pages: 111-141
  • Peer Reviewed
• [Journal Article] Discovery of the strong antioxidant selenoneine in tunaand selenium redox metabolism. (2010)
  • Author(s): Y. Yamashita, T. Yabu, M.
  • Journal Title: World J .Biol. Chem. Volume: 1(5) Pages: 144-150
  • Peer Reviewed
• [Journal Article] Selenoneine,a NovelSelenium-Containing compound, Mediates Detoxification Mechanisms against Methylmercury Accumulation and Toxicity in Zebrafish Embryo.
  • Author(s): M. Yamashita, Y. Yamashita, T. Suzuki, Y. Kani, N. usawa, S. Imamura, K. Takemoto, T. Hara, Md. A. Hossain, T Yabu K. Touhata.
  • Journal Title: Mar.Biotechnol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Origin identification method by multiple trace elemental analysis of intermuscular bones in grilled eel products produced in Japan China, and Taiwan.
  • Author(s): J. Iguchi, Y. Yamashita, K. Touhata, T . Y a b u , M. Yamashita.
  • Journal Title: Fish. Sci . Volume: (in press)
  • Peer Reviewed
• [Presentation] JNK のリン酸化による中性スフィンゴミエ リナーゼ1 の活性化Activation of Neutral Sphingomyelinase 1 by JNK kinase-mediated Phosphorylation (2012)
  • Author(s): 藪健史,司馬肇,柴康宏,中西照幸,今村 伸太朗,山下倫明
  • Organizer: 日本生化学会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 20121214-20121216
• [Book] Heat Stress: Causes, Treatment and Prevention. (2011)
  • Author(s): T. Y abu, S. Imamura, M. Yamashita
  • Total Pages: 215-234
  • Publisher: Nova Science Publishers, Inc.
• [Patent(Industrial Property Rights)] リン酸化中性スフィンゴミエリナーゼ1,抗リン酸化中性スフィンゴミエリナーゼ1抗体,中性スフィンゴミエリナーゼ1変異体,及びそれらの用途。 (2012)
  • Inventor(s): 山下倫明,今村伸太朗,藪健史,東畑顕,石原賢司
  • Industrial Property Rights Holder: 山下倫明,今村伸太朗,藪健史,東畑顕,石原賢司
  • Industrial Property Number: 特許特願2012-272595
  • Filing Date: 2012-12-13