Development of functional foods and feeds using probiotic lactic acid bacteria DNA

2011 Fiscal Year Final Research Report

• Project/Area Number: 22780239
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Zootechnical science/Grassland science
• Research Institution: Shinshu University
• Principal Investigator: SHIMOSATO Takeshi 信州大学, ファイバーナノテク国際若手研究者育成拠点, 助教 (00467200)
• Project Period (FY): 2010 – 2011
• Keywords: 乳酸菌 / オリゴヌクレオチド / 機能性食品 / 機能性飼料

Research Abstract

We found the strong immunostimulatory effects of an oligodeoxynucleotide from lactic acid bacteria, designated MsST. We show that 24-hour of stimulation with MsST in mouse immune cells strongly induces expression of interleukin(IL)-33, a cytokine in the IL-1 superfamily. We synthesized biodegradable capsule containing oligodeoxynucleotide, which showed sustaining-hydrolyzed and preferably degraded in acidic condition. Thereby, we could extend our studies in the oral administrative inhaled approach in intestinal inflammation study.

Research Products

• [Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway (2012)
  • Author(s): Shimazu T, Villena J, Tohno M, Fujie H, Hosoya S, Shimosato T, Aso H, Suda Y, Kawai Y, Saito T, Alvarez S, Ikegami S, Itoh H, Kitazawa H
  • Journal Title: Infection and Immunity Volume: 80(1) Pages: 276-288
  • DOI: DOI:10.1128/IAI.05729-11
• [Journal Article] Immunobiotic Lactobacillus strains augment NLRP3 expression in newborn and adult porcine gut-associated lymphoid tissues (2011)
  • Author(s): Tohno M, Shimosato T, Aso H, Kitazawa H
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 144(3-4) Pages: 410-416
  • DOI: DOI:10.1016/j.vetimm.2011.09.010
• [Journal Article] Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I : C) in porcine intestinal epithelial cells (2011)
  • Author(s): Hosoya S, Villena J, Shimazu T, Tohno M, Fujie H, Chiba E, Shimosato T, Aso H, Suda Y, Kawai Y, Saito T, Alvarez S, Ikegami S, Itoh H, Kitazawa H
  • Journal Title: Veterinary Research Volume: 42(1) Pages: 111
  • DOI: DOI:10.1186/1297-9716-42-111
• [Journal Article] Toll-like receptor-2 activating bifidobacteria strains differentially regulate inflammatory cytokines in porcine intestinal epithelial cell culture system : finding new anti-inflammatory immunobiotics (2011)
  • Author(s): Fujie H, Villena J, Tohno M, Morie K, Shimazu T, Aso H, Suda Y, Shimosato T, Iwabuchi N, Xiao JZ, Yaeshima T, Iwatsuki K, Saito T, Numasaki M, Kitazawa H
  • Journal Title: FEMS Immunology and Medical Microbiology Volume: 63(1) Pages: 129-139
  • DOI: DOI:10.1111/j.1574-695X.2011.00837.x.
• [Journal Article] Effect of Lactobacillus pentosus ONRIC b0240 on intestinal IgA production in mice fed differing levels of protein (2011)
  • Author(s): Shimosato T, Tomida K, Otani H
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 59(6) Pages: 2646-2651
  • DOI: DOI:10.1021/jf104240d
• [Journal Article] Anti-allergic effects of a mixture of Saccharomyces cerevisiae and its specific goat' s milk immunoglobulin G-rich fraction on ovalbuminsensitized BALB/c mice (2011)
  • Author(s): Sekimura Y, Shimosato T, Otani H
  • Journal Title: Milchwissenshaft-Milk Science International Volume: 66(1) Pages: 7-10
  • URL: http://apps.webofknowledge.com/full_record.do?product=UA & search_mode=GeneralSearch & qid=1 & SID=S1FKFnO8ciBDceNcl3L & page=1 & doc=6
• [Journal Article] Accelerated Wound Healing Mediated by Activation of Toll-like Receptor 9 (2010)
  • Author(s): Sato T, Yamamoto M, Shimosato T, KlinmanDM
  • Journal Title: Wound Repair and Regeneration Volume: 18(6) Pages: 586-593
  • DOI: DOI:10.1111/j.1524-475X.2010.00632.x.
• [Journal Article] イムノジェニクスとしての免疫刺激性DNA研究の最前線 (2010)
  • Author(s): 下里剛士, 北澤春樹
  • Journal Title: 北信越畜産学会報 Volume: 100 Pages: 1-9
• [Presentation] 乳酸菌由来オリゴヌクレオチドによる新規分泌型IL-33様タンパク質の誘導 (2012)
  • Author(s): 下里剛士,伊藤雄亮,遠野雅徳,大谷元,北澤春樹
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120327-29
• [Presentation] ビール酵母とその牛乳抗体のマウスI型アレルギーに及ぼす影響 (2012)
  • Author(s): 森崎健太郎,松田孝裕,岡田実,堀江健二,金武祚,下里剛士,大谷元
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120327-29
• [Presentation] ウシ後期初乳の新規生体防御機能の探索 (2012)
  • Author(s): 長谷川実希,遠見和幸,内田健志,為定誠,下里剛士,大谷元
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120327-29
• [Presentation] ウシ後期初乳中の抗アレルギータンパク質の探索 (2012)
  • Author(s): 遠見和幸,須賀正喜,内田健志,為定誠,下里剛士,大谷元
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120327-29
• [Presentation] 乳酸菌菌体外多糖の異なるパターン認識受容体を介する免疫調節機能特性 (2012)
  • Author(s): 室伏陽,和知俊,細矢翔一,遠野雅徳,島津朋之,下里剛士,川井泰,齋藤忠夫,橋口健司,吉田睦子,北澤春樹
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120327-29
• [Presentation] Toll-like receptor 4 recognizes extracellular polysaccharide from Lactococcus lactis subsp. cremoris (2011)
  • Author(s): Tohno M, Shimosato T, Aso H, Kitazawa H
  • Organizer: 4th congress of european microbiologists FEMS 2011
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 20110626-30
• [Presentation] CpG oligodeoxynucleotides promote wound healing in airway epithelial cells (2011)
  • Author(s): Sato T, Miyazawa N, Klinman DM, Shimosato T, Takahashi R, Tsukahara T, Hayashi M, Yamamoto M, Tomaru K, Kaneko T, Ishigatsubo Y
  • Organizer: American Thoracic Society Annual Meeting 2011
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 20110513-18
• [Presentation] Suppressive Oligodeoxynucleotide Synergistically Enhances The Antiproliferative Effect Of Anti-cancer Drug In Human Lung Cancer Cells (2011)
  • Author(s): Takahashi R, Sato S, Miyazawa N, Klinman DM, Shimosato T, Hayashi M, Yamamoto M, Tomaru K, Tsukahara T, Kaneko T, Ishigatsubo Y
  • Organizer: American Thoracic Society Annual Meeting 2011
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 20110513-18
• [Presentation] プルーン果実の免疫調節機能の探索 (2011)
  • Author(s): 宮下亮二,唐澤幸司,下里剛士,大谷元
  • Organizer: 2011年度日本栄養食糧学会大会講演要旨
  • Place of Presentation: 東京
  • Year and Date: 20110513-15
• [Presentation] ウシ後期初乳添加飼料で飼育したマウスのアレルギー関連因子に及ぼす影響 (2011)
  • Author(s): 遠見和幸,清水努,内田健志,為定誠,下里剛士,大谷元
  • Organizer: 第60回北信越畜産学会大会
  • Place of Presentation: 長野
  • Year and Date: 2011-11-11
• [Presentation] ビール酵母とそれに特異的な牛乳IgGのマウスI型アレルギーに及ぼす影響 (2011)
  • Author(s): 森崎健太郎,下里剛士,大谷元
  • Organizer: 第60回北信越畜産学会大会
  • Place of Presentation: 長野
  • Year and Date: 2011-11-11
• [Presentation] Streptococcus thermophilus由来オリゴヌクレオチドにより誘導されるIL-33活性化機構の解析 (2011)
  • Author(s): 下里剛士,伊藤雄亮,遠野雅徳,大谷元,北澤春樹
  • Organizer: 平成23年度日本酪農科学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2011-09-22
• [Presentation] αS1カゼインを発現するLactococcus lactis組み換え体の構築 (2011)
  • Author(s): 下里剛士,重盛駿,大谷元
  • Organizer: 平成23年度日本酪農科学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2011-09-22
• [Presentation] 生体防御に関わるペプチドグリカン認識タンパク質ファミリーの腸管における発現解析 (2011)
  • Author(s): 遠野雅徳,植田渉,島津朋之,下里剛士,麻生久,北澤春樹
  • Organizer: 平成23年度日本酪農科学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2011-09-22
• [Presentation] ウシ腸管上皮細胞による抗ウィルス性イムノバイオティクスの評価系構築 (2011)
  • Author(s): 千葉絵里子,細矢翔一,高梨直也,下里剛士,遠野雅徳,麻生久,川井泰,齋藤忠夫,北澤春樹
  • Organizer: 平成23年度日本酪農科学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2011-09-22
• [Presentation] Streptococcus thermophilus由来オリゴヌクレオチド刺激によるIL-33の断片化解析 (2011)
  • Author(s): 下里剛士,伊藤雄亮,遠野雅徳,大谷元,北澤春樹
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田
  • Year and Date: 2011-08-26
• [Presentation] ウシ後期初乳および常乳のマウスI型アレルギーに及ぼす影響 (2011)
  • Author(s): 遠見和幸,清水努,内田健志,為定誠,下里剛士,大谷元
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田
  • Year and Date: 2011-08-26
• [Presentation] 腸管免疫系におけるイムノバイオティック乳酸菌によるNLRP3受容体の発現増強 (2011)
  • Author(s): 遠野雅徳,下里剛士,麻生久,北澤春樹
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田
  • Year and Date: 2011-08-26
• [Presentation] ウシ腸管上皮細胞による抗ウイルス性イムノバイオティクスの評価系構築 (2011)
  • Author(s): 千葉絵里子,細矢翔一,高梨直也,下里剛士,遠野雅徳,麻生久,川井泰,齋藤忠夫,北澤春樹
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田
  • Year and Date: 2011-08-26
• [Presentation] The novel immunostimulatory activities of CpG oligodeoxynucleotides from Streptococcus thermophilus (2010)
  • Author(s): Shimosato T, Fujimoto M, Tohno M, SatoT, Otani H, Kitazawa H
  • Organizer: ADSA-PSA-AMPA-CSAS-ASAS Joint annual meeting
  • Place of Presentation: CO, USA.
  • Year and Date: 20100711-15
• [Presentation] Effect of immunobiotics on the immune performance and productivity of pigs (2010)
  • Author(s): Suda Y, Takahashi Y, Tohno M, Villena Julio, Hosoya S, Fujie H, Shimosato T, Kobashi J, Ishida M, Aso H, Kawai Y, Saito T, Makino S, Ikegami S, Kitazawa H
  • Organizer: International Scientific Conference on Probiotics and Prebiotics-IPC2010
  • Place of Presentation: Kosice, Slovakia
  • Year and Date: 20100615-17
• [Presentation] Evaluation of Anti-Inflammatory Immunobiotics in Porcine Intesinal Epithelial Cells (2010)
  • Author(s): Kitazawa H, Shimazu T, Tohno M, Villena J, Hosoya S, Fujie H, Suda Y, Shimosato T, Aso H, Makino S, Ikegami S, Kawai Y, Saito T
  • Organizer: International Scientific Conference on Probiotics and Prebiotics-IPC2010
  • Place of Presentation: Kosice, Slovakia
  • Year and Date: 20100615-17
• [Presentation] 乳酸菌由来CpG DNAによるToll様受容体9を介するIL-33の発現増強 (2010)
  • Author(s): 下里剛士,遠野雅徳,大谷元,北澤春樹
  • Organizer: 日本食品免疫学会第6回学術集会
  • Place of Presentation: 東京
  • Year and Date: 20100601-02
• [Presentation] Accelerated wound healing in mice by local administration of sustained-release CpG oligodeoxynucleotide (2010)
  • Author(s): Sato T, Shimosato T, Klinman DM
  • Organizer: 97th AAI Annual Meeting, IMMUNOLOGY 2010
  • Place of Presentation: Baltimore, MD, USA
  • Year and Date: 20100507-11
• [Presentation] Streptococcusthermophilus由来CpG DNAが誘導するIL-33の機能性解析 (2010)
  • Author(s): 下里剛士,畑野木の実,藤本恵,遠野雅徳,大谷元,北澤春樹
  • Organizer: 平成22年度日本酪農科学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-08-20
• [Book] アジュバント開発研究の新展開第三章アジュバント各論「プロバイオティック乳酸菌を利用した経口ワクチン・アジュバント研究の新展開」 (2011)
  • Author(s): 石井健,青枝大貴,小檜山康司,鉄谷耕平,審良静男,山西弘一,吉田裕樹,原博満,安田好文,中西憲司,竹内理,荒木幸一,黒田悦史,小山正平,藤本ゆかり,深瀬浩一,新川武,宮田健,柳義和,豊永憲司,山崎晶,下里剛士,北澤春樹, CevayirCoban, KeiichiOhata, YoshikatsuIgai, MasahiroKato, ToshihiroTsukui,望月慎一,櫻井和朗,岩田晃,前山順一,山本三郎,改正恒康,西川元也,高倉喜信,角田慎一,堤康央,吉開泰信,内田哲也,赤木隆美,明石満,長谷川秀樹,植松智,佐々木津, NathalieGarcon,瀬谷司,佐藤治子,志馬寛明,松本美佐子
  • Total Pages: 100-106
  • Publisher: 株式会社シーエムシー出版