2011 Fiscal Year Final Research Report
Functional analysis of the de novo DNA methyltranserase specific for in vitro-manipulated embryos
| Project/Area Number |
22780248
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
HORII Takuro 群馬大学, 生体調節研究所, 助教 (00361387)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | エピジェネティクス / メチル化 / 受精卵 / 体外操作 |
|---|
| Research Abstract |
In vitro-manipulated embryos, such as in vitro fertilization (IVF), in vitro culture (IVC) embryos often exhibit abnormal DNA methylation patterns in imprinted genes and centromeric and pericentromeric satellite repeats. In this study, we found an alternatively spliced variant of Dnmt3b lacking exon 5 (Dnmt3b・5) that is specific to mouse IVC embryos. In vitro methylation activity assays showed that Dnmt3b・5 had lower activity than normal Dnmt3b. Our findings suggest that Dnmt3b・5 could induce a hypo methylation status especially in vitro manipulated embryos.
|
