2011 Fiscal Year Final Research Report
The study for development of in vitro culture system of mammalian oocyte by global analysis of cumulus cell-stimulating substrate during ovulating process
| Project/Area Number |
22780251
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | Prefectural University of Hiroshima |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 繁殖 / 卵子成熟 / 排卵 |
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| Research Abstract |
During ovulation phase, granulosa cells-derived secretion factors stimulate cumulus cells, which result in completion of oocyte maturation. Since current culture system of oocyte employs only cultivation of cumulus-oocyte complexes(COCs), rate of oocyte maturation is low compared with that of oocyte collected from in vivo due to lack or insufficient of granulosa cells-derived oocyte maturation factors. In this research, we showed that TACE/ADAM17 expressed in granulosa cells and cumulus cells cleaved EGF-like factors from granulosa cells and cumulus cells, which resulted in induction of oocyte maturation via EGFR-ERK1/2 pathway in cumulus cells during ovulation process. We also investigated that PGE2-PTGER2, 4 pathways enhanced expressions of TACE/ADAM17 and EGF-like factors, thereby sustained EGFR-ERK1/2 pathway in cumulus cells during oocyte maturation. Furthermore, c-Src phosphorylated by FSH-PKC stimuli induced association with TACE/ADAM17, which required for protease activity of TACE/ADAM17 in granulosa cells and cumulus cells. Finally, optimization of expression and activation pattern of TACE/ADAM17 in cumulus cells of COCs by PGE2 or FSH-c-Src improved maturation rate of oocyte during in vitro maturation of porcine COCs
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[Book] 卵子学2011
Author(s)
島田昌之, 山下泰尚
Total Pages
818-829
Publisher
京都大学出版会
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