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2011 Fiscal Year Final Research Report

The study for development of in vitro culture system of mammalian oocyte by global analysis of cumulus cell-stimulating substrate during ovulating process

Research Project

  • PDF
Project/Area Number 22780251
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionPrefectural University of Hiroshima

Principal Investigator

YAMASHITA Yasuhisa  県立広島大学, 生命環境学部, 准教授 (50452545)

Project Period (FY) 2010 – 2011
Keywords繁殖 / 卵子成熟 / 排卵
Research Abstract

During ovulation phase, granulosa cells-derived secretion factors stimulate cumulus cells, which result in completion of oocyte maturation. Since current culture system of oocyte employs only cultivation of cumulus-oocyte complexes(COCs), rate of oocyte maturation is low compared with that of oocyte collected from in vivo due to lack or insufficient of granulosa cells-derived oocyte maturation factors. In this research, we showed that TACE/ADAM17 expressed in granulosa cells and cumulus cells cleaved EGF-like factors from granulosa cells and cumulus cells, which resulted in induction of oocyte maturation via EGFR-ERK1/2 pathway in cumulus cells during ovulation process. We also investigated that PGE2-PTGER2, 4 pathways enhanced expressions of TACE/ADAM17 and EGF-like factors, thereby sustained EGFR-ERK1/2 pathway in cumulus cells during oocyte maturation. Furthermore, c-Src phosphorylated by FSH-PKC stimuli induced association with TACE/ADAM17, which required for protease activity of TACE/ADAM17 in granulosa cells and cumulus cells. Finally, optimization of expression and activation pattern of TACE/ADAM17 in cumulus cells of COCs by PGE2 or FSH-c-Src improved maturation rate of oocyte during in vitro maturation of porcine COCs

  • Research Products

    (18 results)

All 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (9 results) Book (1 results) Remarks (1 results)

  • [Journal Article] The key signaling cascades in granulosa cells during follicular development and ovulation process2011

    • Author(s)
      Shimada M, Yamashita Y
    • Journal Title

      Journal of Mammalian Ova Research

      Volume: 28 Pages: 25-31

    • Peer Reviewed
  • [Journal Article] Cryopreservation of microencapsulated canine sperm2011

    • Author(s)
      Sha S, Otsuki T, Fujiwara C, Yamamoto N, Yamashita Y, Higaki S, Hishinuma M
    • Journal Title

      Theriogenology

      Volume: 75 Pages: 679-686

    • Peer Reviewed
  • [Journal Article] Effect of butylated hydroxytikuene on dog sperm longevity in chilling storage and cryopreservation2011

    • Author(s)
      Sahashi Y, Otsuki T, Higaki S, Nagano M, Yamashita Y, Hishinuma M
    • Journal Title

      Journal of Veterinary Medicine Science

      Volume: 73 Pages: 1355-1357

    • Peer Reviewed
  • [Journal Article] Positive feedback loop between prostaglandin E2 and EGF-like factors is essential of the sustainable activation of MAPK3/1 in cumulus cells during in vitro maturation of porcine cumulus-oocyte complexes2011

    • Author(s)
      Yamashita Y, Okamoto M, Kawashima I, Nishmura R, Gunji Y, Hishinuma M, Shimada M
    • Journal Title

      Biology of Reproduction

      Volume: 85 Pages: 1073-1082

    • Peer Reviewed
  • [Journal Article] Progesterone is essential for maintenance of Tace/Adam17 mRNA expression, but not EGF-like factor in cumulus cells, which enhances the EGF receptor signaling pathway during in vitro maturation of porcine COCs2010

    • Author(s)
      Yamashita Y, Kawashima I, Gunji Y, Nishimura R, Hishinuma M, Shimada M
    • Journal Title

      Journal of Reproduction and Development

      Volume: 56 Pages: 315-323

    • Peer Reviewed
  • [Journal Article] Microencapsulation of canine sperm and its preservation at 4 degree C2010

    • Author(s)
      Shah S, Nagano M, Yamashita Y, Hishinuma M
    • Journal Title

      Theriogenology

      Volume: 73 Pages: 560-567

    • Peer Reviewed
  • [Journal Article] The release of EGF domain from EGF-like factors by specific cleavage enzyme activates EGFR-MAPK3/1 pathway in both granulosa cells and cumulus cells during ovulation process

    • Author(s)
      Yamashita Y, Shimada M
    • Journal Title

      Journal of Reproduction and Development

      Volume: (in press)

    • Peer Reviewed
  • [Presentation] EGF-like factor-ADAM17 networkにより誘導される哺乳動物卵の成熟機構の解析と応用2012

    • Author(s)
      山下泰尚, 菱沼貢, 島田昌之
    • Organizer
      第153回日本獣医学会
    • Place of Presentation
      埼玉県
    • Year and Date
      2012-03-28
  • [Presentation] 卵胞液中ホルモン濃度の経時的変化を模倣した牛卵子体外培養法の開発2011

    • Author(s)
      郡司庸介, 武井直樹, 渡邊貴之, 光藤弘俊, 西村亮, 山下泰尚, 檜垣彰吾, 鈴木稔, 菱沼貢
    • Organizer
      第152回日本獣医学会
    • Place of Presentation
      大阪府
    • Year and Date
      2011-09-21
  • [Presentation] 牛卵巣における黄体の有無が体外胚の生産に及ぼす影響2011

    • Author(s)
      光藤弘俊, 郡司庸介, 西村亮, 山下泰尚, 檜垣彰吾, 菱沼貢
    • Organizer
      第152回日本獣医学会
    • Place of Presentation
      大阪府
    • Year and Date
      2011-09-21
  • [Presentation] 犬精子の凍結保存における凍結保存液の浸透圧とグリセリン濃度の検討2011

    • Author(s)
      藤村千加, 檜垣彰吾, 郡司庸介, 大月翼, 泉千尋, 岡崎哲司, 島田昌之, 山下泰尚, 菱沼貢
    • Organizer
      第152回日本獣医学会
    • Place of Presentation
      大阪府
    • Year and Date
      2011-09-20
  • [Presentation] 牛卵胞および黄体におけるBNIP3の発現2011

    • Author(s)
      西村亮, 奥田潔, 郡司庸介, 光藤弘俊, 檜垣彰吾, 山下泰尚, 菱沼貢
    • Organizer
      第152回日本獣医学会
    • Place of Presentation
      大阪府
    • Year and Date
      2011-09-20
  • [Presentation] 哺乳動物卵子の成熟・排卵機構の基礎的解析とその応用2011

    • Author(s)
      山下泰尚, 菱沼貢, 島田昌之
    • Organizer
      第151回日本獣医学会
    • Place of Presentation
      東京都
    • Year and Date
      2011-03-31
  • [Presentation] Crypereservation of microencapcelated canine sperm2010

    • Author(s)
      Shah S, 大月翼, 藤村千加, 山本直樹, 山下泰尚, 檜垣彰吾, 菱沼貢
    • Organizer
      第150回日本獣医学会
    • Place of Presentation
      帯広市
    • Year and Date
      2010-09-16
  • [Presentation] 鳥取県内に生息する野生ヌートリアの繁殖生理2010

    • Author(s)
      伊從留南, 山下泰尚, 檜垣彰吾, 菱沼貢
    • Organizer
      第44回鳥取県獣医学会
    • Place of Presentation
      鳥取市
    • Year and Date
      2010-07-17
  • [Presentation] ブタ顆粒膜細胞において, PKCとc-Src依存的なTACE./ADAM17の活性化が, EGF-like factorの分泌を介して顆粒膜細胞の黄体会,卵丘膨化,および卵成熟を促進する2010

    • Author(s)
      山下泰尚, 岡本美奈子, 菱沼貢, 島田昌之
    • Organizer
      第52回日本哺乳動物卵子学会
    • Place of Presentation
      栃木県
    • Year and Date
      2010-05-21
  • [Book] 卵子学2011

    • Author(s)
      島田昌之, 山下泰尚
    • Total Pages
      818-829
    • Publisher
      京都大学出版会
  • [Remarks]

    • URL

      http://www.pu-hiroshima.ac.jp/10_research/01_researcher/post_392/post_532/index.ht

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Published: 2013-07-31  

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