2013 Fiscal Year Final Research Report
Identification and evaluation of sperm surface molecules involved in the molecular mechanisms of avian egg-sperm interactions
| Project/Area Number |
22780255
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | Meijo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010-04-01 – 2014-03-31
|
| Keywords | 受精 / 卵膜 / 透明帯 / ZP 糖タンパク質 / タンパク質間相互作用 / マトリックス形成 |
|---|
| Research Abstract |
Vertetbrate egg cell is covered with a proteinous membrane called egg coat or zona pellucida. The egg coat being composed of 3-5 classes of ZP glycoproteins play important roles in fertilization to accept and activate sperm followed by the successful fusion with egg. We are studying on chicken egg coat and contributed to solve 3D structure of one of the ZP glycoproteins, ZP3, which is involved in the egg-sperm recognition. Furthermore, we revealed the sperm binding site on the ZP3 molecule using recombinant proteins based on the structural information of ZP3. In addition, we revealed that the isoforms of chicken ZP3 possess different types of sugar chains each other.
|
-
-
-
-
-
-
-
[Presentation] 組換えZPタンパク質を用いたニワトリ卵膜形成機構の解明2012
Author(s)
Yurie Ima, Hideaki Fukushima, Minoru Ujita and Hiroki Okumura
Organizer
The 85th Meeting of the Japanese Biochemical Society
Place of Presentation
Fukuoka, Japan
Year and Date
20121214-16
-
-
-
-
-
[Presentation] ニワトリ卵膜形成機構の解析-血清ZP1の卵膜への組込み機構-2011
Author(s)
Msaki Momoda, Yurie Ima, Daishi Okada, Minoru Ujita and Hiroki Okumura
Organizer
The 84 th Meeting of the Japanese Biochemical Society
Place of Presentation
Kyoto, Japan
Year and Date
20110921-24
-
-
-
-
[Presentation] 組換えZPタンパク質を用いた鳥類卵膜の機能解析および構造解析2010
Author(s)
Daishi Okada, Masaki Momoda, Mayumi Arai, Minoru Ujita and Hiroki Okumura
Organizer
The Joint Meeting of the Japanese Biochemical Society & the Molecular Biology Society of Japan; BMB2010
Place of Presentation
Kobe, Japan
Year and Date
20101207-10
-
[Presentation] 組換えヒトDectin-1のβ-グルカン結合特異性2010
Author(s)
Yohsuke Yamauchi, Syota Koike, Takayo Funato, Satoko Kanie, Hiroki Okumura and Minoru Ujita
Organizer
The Joint Meeting of the Japanese Biochemical Society & the Molecular Biology Society of Japan; BMB2010
Place of Presentation
Kobe, Japan
Year and Date
20101207-10
-
[Presentation] 組換えヒトキチナーゼの結合活性2010
Author(s)
Masahiro Yamada, Mayumi Yamanaka, Miyuki Haruta, Hiromi Umino, Yuka Itakura, Maika Mikuriya, Saori Kato, Hiroki Okumura and Minoru Ujita
Organizer
The Joint Meeting of the Japanese Biochemical Society & the Molecular Biology Society of Japan; BMB2010
Place of Presentation
Kobe, Japan
Year and Date
20101207-10
-
-
[Remarks] Hiroki Okumura. X-ray structure of ZP3 provides clues to elucidate molecular mechanisms of egg coat (zona pellucida) assembly and egg-sperm interaction. Special Lecture Tokuron FY2010 (Mar. 4, 2011, Graduate School of Medicine, Nagoya University, Japan)
