High resolution transcriptome analysis in protozoan parasites

2012 Fiscal Year Final Research Report

• Project/Area Number: 22780258
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Tohoku University (2012); Obihiro University of Agriculture and Veterinary Medicine (2010-2011)
• Principal Investigator: YAMAGISHI Junya 東北大学, 東北メディカル・メガバンク機構, 助教 (80535328)
• Project Period (FY): 2010 – 2012
• Keywords: 原虫 / トキソプラズマ / トランスクリプトーム / 次世代シーケンサー / 転写開始点 / バイオインフォマティクス

Research Abstract

By using RNA-seq and TSS-seq which are one of the application of next generation sequencers, it becomes possible to obtain transcriptomes of wide variety of living organisms. We applied the methods on representative three stages which consist of lifecycle of an obligate, intracellular, parasitic protozoan, Toxoplasma gondii. As a result, we demonstrated gene expression profiles of the three stages as well as putative DNA cis-elements which regulate the gene expression.

Research Products

• [Journal Article] Characterization of Toxoplasma gondii5' UTR with encyclopedic TSS information (2012)
  • Author(s): Yamagishi J, Watanabe J, Goo YK, Masatani T, Suzuki Y, Xuan X
  • Journal Title: J Parasitol Volume: 98(2) Pages: 445-7
  • DOI: doi:10.1645/GE-2864.1
  • Peer Reviewed
• [Journal Article] Construction and analysis of full-length cDNA library of Cryptosporidium parvum (2011)
  • Author(s): Yamagishi J, Wakaguri H, Sugano S, Kawano S, Fujisaki K, Sugimoto C, Watanabe J, Suzuki Y, Kimata I, Xuan X
  • Journal Title: Parasitol Int Volume: 60(2) Pages: 199-202
  • DOI: doi:10.1016/j.parint.2011.03.001
  • Peer Reviewed
• [Journal Article] Full-parasites: database of full-length cDNAs of apicomplexa parasites, 2010 update (2011)
  • Author(s): Tuda J, Mongan AE, Tolba ME,Imada M, Yamagishi J, Xuan X, Wakaguri H, Sugano S, Sugimoto C, Suzuki Y. Nucleic
  • Journal Title: Acids Res Volume: 39(Database issue) Pages: D625-31
  • DOI: doi:10.1093/nar/gkq1111
  • Peer Reviewed
• [Journal Article] High-resolution characterization ofToxoplasma gondii transcriptome with a massive parallel sequencing method (2010)
  • Author(s): Yamagishi J, Wakaguri H, Ueno A, Goo YK, Tolba M, Igarashi M, Nishikawa Y, Sugimoto C, Sugano S, Suzuki Y,Watanabe J, Xuan X
  • Journal Title: DNA Res Volume: 17(4) Pages: 233-43
  • DOI: doi:10.1093/dnares/ dsq013
  • Peer Reviewed
• [Presentation] Advances in Tropical Genomics: Conservation and Sustainable Utilization of Tropical Biodiversity (2011)
  • Author(s): Junya Yamagishi, Hiroyuki Wakaguri,Junichi Watanabe, Kawazu Shin-ichiro, Yutaka Suzuki, Xuenan Xuan
  • Organizer: Transcriptomics of a Parasit Toxoplasma gondii from Next Generation Sequencing
  • Place of Presentation: Bogor, Indonesia
  • Year and Date: 20111220-21
• [Presentation] XIII International Congress ofBacteriology and Applied Microbiology (2011)
  • Author(s): Junya Yamagishi, Hiroyuki Wakaguri, Akio Ueno, Makoto Igarashi, Junichi Watanabe, Yutaka Suzuki, Xuenan Xuan
  • Organizer: Comparative transcriptomics among tachyzoites, bradyzoites and sporozoites of Toxoplasma gondii using massive parallel sequencing method
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20110906-10
• [Remarks] Full-Toxoplasma
  • URL: http://fullmal.hgc.jp/index_tg_ajax.html