2011 Fiscal Year Final Research Report
Role of nucleocapsid protein of Mononegaviruses in evasion of innate immunity
| Project/Area Number |
22780261
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Gifu University |
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Principal Investigator |
ITO Naoto 岐阜大学, 応用生物科学部, 准教授 (20334922)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 狂犬病ウイルス / マイナス鎖RNAウイルス / ヌクレオカプシド / 自然免疫 / インターフェロン / RIG-I |
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| Research Abstract |
Rabies virus, by a function of its nucleocapcid(N) protein, evades activation of the cellular factor RIG-I, which recognizes viral RNA and plays important role in induction of type I interferon. The aim of this study was to elucidate the molecular mechanism underlying this phenomenon. We found that amino acids at positions 273 and 394 in N protein are important for evasion of RIG-I activation. Also, we determined molecular structure of defective interfering(DI) RNAs, which is a possible target for RIG-I, and established molecular basis to quantify DI RNAs.
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