Molecular basis of the tick embryo parasitized by Babesia protozoa

2012 Fiscal Year Final Research Report

• Project/Area Number: 22780273
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: HATTA Takeshi 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所・細菌・寄生虫研究領域, 研究員 (00455304)
• Project Period (FY): 2010 – 2012
• Keywords: フタトゲチマダニ / Babesia gibsoni / バベシア原虫 / 介卵伝播 / マイクロアレイ

Research Abstract

Tick is a hematophagous arthropod which transmits canine Babesia protozoa, Babesia gibsoni. In this, the protozoa which taken by the parental ticks during the blood-feeding, shifts to next-generation larval ticks through an egg (transovarial transmission). In this research, two experimental systems including transovarial transmission and microarray analysis were established to analyze the gene expression profile of tick embryo parasitized by B. gibsoni. Here, we assumed that the expression of the genes constitutively expressing in the tick might be stable even though they were infected with the parasites.

Research Products

• [Journal Article] Quantitative PCR-based parasite burden estimation of Babesia gibsoni in the vector tick, Haemaphysalis longicornis (Acari: Ixodidae), fed on an experimentally infected dog (2013)
  • Author(s): Hatta T, Matsubayashi M, Miyoshi T,Islam K, Alim MA, Anisuzzaman, Yamaji K, Fujisaki K, Tsuji N
  • Journal Title: J Vet Med Sci Volume: 75(1) Pages: 1-6
  • Peer Reviewed
• [Journal Article] Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis (2012)
  • Author(s): Hatta T, Miyoshi T, Matsubayashi M, Islam MK, Alim MA, Anisuzzaman, Yamaji K, Fujisaki K, Tsuji N
  • Journal Title: Parasit Vectors Volume: 5 Pages: 263
  • DOI: doi:10.1186/1756-3305-5-263
  • Peer Reviewed
• [Presentation] 人工吸血法により作出したバベシア原虫感染マダニ中腸mRNA-seq解析 (2013)
  • Author(s): 八田岳士ら
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 20130329-20130331
• [Presentation] 人工吸血法により作出したバベシア原虫感染マダニ中腸mRNA-seq解析 (2012)
  • Author(s): 八田岳士ら
  • Organizer: 第72回日本寄生虫学会東日本支部会・第10回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 群馬大学
  • Year and Date: 20121012-20121013
• [Presentation] フタトゲチマダニ人工吸血系の確立とBabesia ovata感染マダニ作出への応用 (2012)
  • Author(s): 八田岳士ら
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学
  • Year and Date: 20120914-20120916
• [Presentation] Hypothetical coexistencestrategies of Babesia protozoa and vector ticks: the relationship betweena parasite burden and theblood-digestion in the tick midgut (2011)
  • Author(s): Hatta, et al
  • Organizer: VIIth International Conference on Ticks and Tick-borne Pathogens
  • Place of Presentation: Zaragoza, Spain
  • Year and Date: 20110829-30
• [Presentation] バベシア原虫の介卵伝播に関与するマダニ発育胚の分子基盤について (2010)
  • Author(s): 八田岳士ら
  • Organizer: 第9回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 長崎大学
  • Year and Date: 2010-10-08