2012 Fiscal Year Final Research Report
Study on the molecular basis behind chicken colonization of Campylobacter jejuni
| Project/Area Number |
22780275
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
ASAKURA Hiroshi 国立医薬品食品衛生研究所, 食品衛生管理部, 室長 (40370936)
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| Project Period (FY) |
2010 – 2012
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| Keywords | カンピロバクター / 獣医公衆衛生 |
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| Research Abstract |
Campylobacter jejuni is a leading cause of foodborne illness worldwide. In this study, GFP-expressing C. jejuni was constructed, which was then inoculated orally to 2week-old-chicken. After 0, 1, and 4 weeks post infection, cecum contents were collected and GFP-positive bacterial cells were selectively recovered through FACS sorting. The collected samples were then used to iTRAQ-labelling, thereby being subjected to LC-MS/MS analyses. Comparative analyses revealed that 55 proteins were consistently detected from all samples, in which three samples showed increased amounts in time-dependently after chicken inoculation. Mutation of fabG that encodes putative 3-ketoacyl-(acyl-carrier-protein) reductase, impaired cell adhesion whereas did not affect bacterial motility, suggesting the functional role of this mutation in interaction to host cell. Fatty acid composition analysis revealed the significant decrease of the oleic acids in the fabG mutant, compared with the parental strain, providing an idea that such fatty acid compositions may affect host-pathogen interaction, altering intestinal colonization of this pathogen in chicken.
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[Journal Article] Evaluation of the culture method NIHSJ-02 alternative to ISO 10272-1:2006 for the detection of Campylobacter jejuni and Campylobacter coli in chicken: Collaborative study2013
Author(s)
Momose Y, Okada Y, Asakura H, Ekawa T, Masuda K, Matsuoka H, Yokoyama K, Kai A, Saito S, Hiramatsu R, Taguchi M, Ishimura K, Tominaga K, Yahiro S, Fujita M, Igimi S
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Journal Title
Journal of AOAC International
Peer Reviewed
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[Journal Article] Helicobacter pylori HopQ identified as a novel T4SS-associated virulence factor2013
Author(s)
Belogolova E, Bauer B, Pompaiah M, Asakura H, Brinkmann V, Ertl C, Bartfeld S, Nechitaylo T, Haas R, Machuy N, Salama N, Churin Y, Meyer TF
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Journal Title
Peer Reviewed
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[Journal Article] Prevalence and growth kinetics of Shiga toxin-producing Escherichia coli (STEC) in bovine offal products in Japan2012
Author(s)
Asakura H, Saito E, Momose Y, Ekawa T, Sawada M, Yamamoto A, Hasegawa A, Iwahori J, Tsutsui T, Osaka K, Matsushita T, Kakinuma M, Motoyama K, Hayama Y, Kitamoto H, Igimi S, Kasuga F
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Journal Title
Epidemiol. Infect.
Volume: 140(4)
Pages: 655-664
DOI
Peer Reviewed
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[Journal Article] Helicobacter pylori HP0518 affects flagellin glycosylation to alter bacterial motility2010
Author(s)
Asakura H, Churin Y, Bauer B, Boettcher JP, Bartfeld S, Hashii N, Kawasaki N, Mollenkopf HJ, Jungblut PR, Brinkmann V, Meyer TF
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Journal Title
Molecular Microbiology
Volume: 78(5)
Pages: 1130-1144
DOI
Peer Reviewed
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[Presentation] Antimicrobial susceptibility profiles and PFGE typing of Campylobacter jejuni and their implications to public health in Japan2012
Author(s)
Igimi S, Ishiwa A, Monden S, Okada Y, Asakura H, Momose Y, Asai T, Kai A, Yokoyama K, Taguchi M, Ishii Y, Kuroda M, and Watanabe H
Organizer
UJNR-46^<th> Toxic Microorganisms Joint Panel Meeting
Place of Presentation
東京
Year and Date
20120300
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[Presentation] Antimicrobial susceptibility profiles and PFGE typing of Campylobacter jejuni isolated from various sources in Japan2011
Author(s)
Igimi S, Ishiwa A, Monden S, Okada Y, Asakura H, Asai T, Kai A, Yokoyama K, Taguchi M, Ishii Y, Kuroda M, Watanabe H
Organizer
16^<th> International Symposium on Campylobacter, Helicobacter and its related organisms (CHRO2011)
Place of Presentation
Vancouver, Canada
Year and Date
20110800
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