2011 Fiscal Year Final Research Report
Basic and clinical investigation of the mechanism in the aldosterone regulation by the sympathetic system
| Project/Area Number |
22780284
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Clinical veterinary science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 内科 / 心臓病 / アルドステロン / 線維化 |
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| Research Abstract |
Our objectivewas to investigate the effectsof doxycycline, a matrix metalloproteinase(MMP) inhibitor(MMPi) on ss-agonist-induced myocardial fibrosis and MMP expression. Rats were divided into 3groups : control, Isoproterenol(ISO), and Isoproterenolwith doxycycline(ISO+DOX). Compared to the control, ISO induced the myocardial hypertrophyand cardiacfibrosis, but were attenuated by co-treatment with DOX. MMP-2 activity alsoincreased in the ISO treatment, but decreased by co-treatment with DOX. Similarly, immunoblotting showedsignificant increaseinMMP-2levelin the ISO, and decreased levels in the DOX co-treatment. Ourresults suggest that the enhanced expression of MMP-2plays a prominent role inpromoting myocardial fibrosis inβ-agonist signaling pathway, and that MMP-inhibiting compoundsmay attenuate myocardial fibrosis.
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