2011 Fiscal Year Final Research Report
Molecular mechanism of meiotic arrest through Mos-MAPK pathway in mouse oocytes
| Project/Area Number |
22780299
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 発生 / 分化制御 |
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| Research Abstract |
Ovulated oocytes are arrested at the metaphase of second meiotic division (metaphase-II). In this study, we showed possible role of mitogen-and stress-activated kinase 1, MSK1 known as a downstream kinase of the Mos-MAPK pathway, in the mouse oocytes. MSK1 exhibited the ability to phosphorylate four Ser/Thr residues of meiotic cell-cycle regulator EMI2. The phosphorylation was required for up-regulation of the EMI2 activity in the oocytes. These results suggested that mouse MSK1 may play a key role in the metaphase-II arrest through phosphorylation of EMI2.
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[Journal Article] Dynamic regulation of Emi2 by Emi2-bound Cdk1/Plk1/CK1 and PP2A-B56 in meiotic arrest of Xenopus eggs2011
Author(s)
Isoda, M., Sako, K., Suzuki, K., Nishino, K., Nakajo, N., Ohe, M., Ezaki, T., Kanemori, Y., Inoue, D., Ueno, H. and Sagata, N
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Journal Title
Developmental Cell
Volume: 13
Pages: 506-519
Peer Reviewed
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[Journal Article] Emi2 inhibition of the Anaphase-promoting Complex/Cyclosome absolutely requires Emi2 binding via the C-terminal RL tail2010
Author(s)
Ohe, M., Kawamura, Y., Ueno, H., Inoue, D., Kanemori, Y., Senoo, C., Isoda, M., Nakajo, N. and Sagata, N
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Journal Title
Molecular Biology of the Cell
Volume: 15
Pages: 905-913
Peer Reviewed
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