2013 Fiscal Year Final Research Report
Study on the molecular basis of environmental stress sensing and response in C. elegans intestinal cells
| Project/Area Number |
22790081
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TOHYAMA Koujiro 岩手医科大学, 医歯薬総合研究所, 教授 (10129033)
OHASHI-KOBAYASHI Ayako 岩手医科大学, 薬学部, 教授 (90272484)
TANJI Takahiro 岩手医科大学, 薬学部, 助教 (60453320)
NISHIKORI Kenji 岩手医科大学, 薬学部, 助教 (20563844)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 線虫 / 腸細胞 / 飢餓 / ペントースリン酸回路 / ABC輸送体 / RNAi / 電子顕微鏡 / 薬学 |
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| Research Abstract |
The intestinal cells of the nematode C. elegans possess enriched non-acidic granules for whose formation ABC transporters HAF-4 and HAF-9 are required. The abundant granules rapidly disappear upon food deprivation, suggesting the existence of unknown mechanisms on nutritional storage and starvation stress response at the organelle level. Light-microscopic and transmission electron microscopic observation of the starvation response revealed the rapid granular deterioration associated with the granular membrane redistribution, which seems to be independent of autophagic membrane degradation. Furthermore, we found that the gene knockdown of several pentose phosphate pathway-related genes causes the loss or abnormal formation of specific subsets of the intestinal granules including the non-acidic ones.
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