2011 Fiscal Year Final Research Report
Construction of Evaluation Model for Adipocyte Hypertrophy in vitro and Study on Elucidation of Mechanism of Adipocyte Enlargement
Project/Area Number |
22790139
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Environmental pharmacy
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Research Institution | Tokushima Bunri University |
Principal Investigator |
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Project Period (FY) |
2010 – 2011
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Keywords | 細胞・組織 / 脂質 / 肥満 |
Research Abstract |
We constructed 3D cell culture system of evaluation model for adipocyte hypertrophy in vitro. As a result, enlarged adipocytes with a unilocular lipid droplet were observed. We also analyzed expression patterns of adipocyte enlargement maker gene, MEST. MEST mRNA was upregulated by increasing intracellular cAMP during adipocyte differentiation. We also found an enhancer region sensitive to the cAMP elevation, which is located 5'-upstream of the MEST promoter.
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