2012 Fiscal Year Final Research Report
Three-dimensional culture of the colony-forming parenchymal hepatocytes and endothelial cells for prediction of drug metabolism
| Project/Area Number |
22790168
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical pharmacy
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| Research Institution | Teikyo Heisei University |
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Principal Investigator |
OHNO Maki 帝京平成大学, 薬学部, 助教 (80366765)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 薬物動態 / 代謝学 |
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| Research Abstract |
Primary human hepatocytes are used extensively to study drug-metabolizing enzymes such as the cytochrome P450 enzymes. However, the activities of these enzymes decrease rapidly during culture. Thus, a more functional culture system is required to obtain the usually high levels of activity and regulation ability of drug metabolizing systems. In the present study, using a thermo-responsive culture dish, layered co-culture was achieved by placing a pulmonary artery endothelial cell sheet onto the human small hepatocyte. The expression levels of drug-metabolizing enzymes were significantly increased, compared with the monolayer cultured small hepatocytes.
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