Construction of the miRNA database associate with chemotherapeutic sensitivity in lung cancer.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790173
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Meiji Pharmaceutical University
• Principal Investigator: SUZUKI Toshihiro 明治薬科大学, 薬学部, 講師 (80322527)
• Project Period (FY): 2010 – 2011
• Keywords: miRNA / バイオマーカー / 肺がん / 薬剤耐性 / ゲフィチニブ

Research Abstract

Gefitinib has been used for treatment of non-small cell lung cancer. The EGFR gene mutation correlates with clinical response to Gefitinib. However, some patients are resistant to Gefitinib despite the existence of mutated EGFR gene. This resistance is a major problem in patients with NSCLC. Sensitive biomarker is required for effective therapy with chemotherapeutic drugs. Recently, circulating tumor cell and microRNA(miRNA) are focused on as biomarkers for cancer diagnosis.
In this study, we utilized miRNA array and real-time PCR to show that some miRNAs are significantly up-regulated in Gefitinib resistant lung cancer cell lines. Using the miRNA specific inhibitors, we identified ErbB family molecules are as a target gene of these miRNAs.

Research Products

• [Journal Article] An in vivo highly antitumor-active tetrazolato-bridged dinuclear platinum(ii) complex largely circumvents in vitro cisplatin resistance : two linkage isomers yield the same product upon reaction with 9-ethylguanine but exhibit different cytotoxic profiles (2012)
  • Author(s): Uemura M, Suzuki T, Nishio K, Chikuma M, Komeda S.
  • Journal Title: Metallomics
  • DOI: DOI:10.1039/C2MT20026K
  • Peer Reviewed
• [Journal Article] Database of the clinical phenotypes, genotypes and mutant arylsulfatase B structures in mucopoly-saccharidosis type VI (2012)
  • Author(s): Saito S, Ohno K, Sekijima M, Suzuki T, Sakuraba H
  • Journal Title: J Hum Genet Volume: 57 Pages: 280-282
  • DOI: DOI:10.1038/jhg.2012.6.
  • Peer Reviewed
• [Journal Article] Anti-angiogenic effect of α-mangostin (2012)
  • Author(s): Shiozaki T, Fukai M, Hermawati E, Juliawaty LD, Syah YM, Hakim EH, Puthongking P, Suzuki T, Kinoshita K, Takahashi K, Koyama K., Takahashi K, Koyama K.
  • Journal Title: J Nat Med
  • DOI: DOI:10.1007/s11418-012-0645-z
  • Peer Reviewed
• [Journal Article] Structural bases of Wolman disease and cholesteryl ester storage disease. (2012)
  • Author(s): Saito S, Ohno K, Suzuki T, Sakuraba H.
  • Journal Title: Mol Genet Metab. Volume: 105 Pages: 244-248
  • Peer Reviewed
• [Journal Article] Hypoxylonols C-F, benzo [j] fluoranthenes from Hypoxylon truncatum. (2012)
  • Author(s): Fukai M, Tsukada M, Miki K, Suzuki T, Sugita T, Kinoshita K, Takahashi K, Shiro M, Koyama K.
  • Journal Title: J Nat Prod. Volume: 75 Pages: 22-25
  • DOI: DOI:10.1021/np2004193
  • Peer Reviewed
• [Journal Article] Efficient uptake of recombinant α-galactosidase A produced with a gene-manipulated yeast by Fabry mice kidneys. (2012)
  • Author(s): Tsukimura T, Kawashima I, Togawa T, Kodama T, Suzuki T, Watanabe T, Chiba Y, Jigami Y, Fukushige T, Kanekura T, Sakuraba H.
  • Journal Title: Mol Med. Volume: 18 Pages: 76-82
  • Peer Reviewed
• [Journal Article] Chemical constituents of a marine fungus, Arthrinium sacchari. (2011)
  • Author(s): Tsukada M, Fukai M, Miki K, Shiraishi T, Suzuki T, Nishio K, Sugita T, Ishino M, Kinoshita K, Takahashi K, Shiro M, Koyama K.
  • Journal Title: J Nat Prod. Volume: 74 Pages: 1645-1649
  • Peer Reviewed
• [Journal Article] Lyso-GM2 ganglioside : a possible biomarker of Tay-Sachs disease and Sandhoff disease. (2011)
  • Author(s): Kodama T, Togawa T, Tsukimura T, Kawashima I, Matsuoka K, Kitakaze K, Tsuji D, Itoh K, Ishida Y, Suzuki M, Suzuki T, Sakuraba H.
  • Journal Title: PLoS One Volume: 6(12) Pages: e29074
  • Peer Reviewed
• [Journal Article] Inhibitor Isolated from the Mushroom Boletopsis leucomelas. (2010)
  • Author(s): Kaneko A, Tsukada M, Fukai M, Suzuki T, Nishio K, Miki K, Kinoshita K, Takahashi K, Koyama K. KDR Kinase
  • Journal Title: J Nat Prod. Volume: 73 Pages: 1002-1004
  • Peer Reviewed
• [Journal Article] Antiangiogenic metabolites from a marine-derived fungus, Hypocrea vinosa. (2010)
  • Author(s): Ohkawa Y, Miki K, Suzuki T, Nishio K, Sugita T, Kinoshita K, Takahashi K, Koyama K
  • Journal Title: J Nat Prod. Volume: 73 Pages: 579-582
  • Peer Reviewed
• [Presentation] miRNAによるゲフィチニブ耐性調節機構の解明 (2012)
  • Author(s): 入澤愛、鈴木俊宏、西尾和人、大森亨、兎川忠靖、櫻庭均
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 20120300
• [Presentation] ゲフィチニブ耐性細胞株におけるmicroRNAの発現解析 (2011)
  • Author(s): 入澤愛、鈴木俊宏、西尾和人、大森亨、兎川忠靖、櫻庭均
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111000
• [Presentation] ゲフィチニブ耐性に関わるmicroRNAの探索 (2011)
  • Author(s): 入澤愛、鈴木俊宏、西尾和人、大森亨、兎川忠靖、櫻庭均
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 20110900
• [Presentation] MicroRNA expression profiling in cisplatin resistant human non-small cell lung cancer cell lines (2011)
  • Author(s): Suzuki T, Irisawa A, Nishio K, Togawa T, Sakuraba H
  • Organizer: American Association for Cancer Research 102nd Annual Meeting
  • Place of Presentation: Orlando, Florida
  • Year and Date: 20110400
• [Presentation] 非小細胞肺がん細胞株におけるmiRNAのプロファイリング (2011)
  • Author(s): 鈴木俊宏、入澤愛、西尾和人、大森亨、兎川忠靖、櫻庭均
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 20110300
• [Presentation] MicroRNA expression profiling in drug resistant lung cancer cell lines (2011)
  • Author(s): Suzuki T, Togawa T, Sakuraba H
  • Organizer: The 1st Medicinal Chemistry Seminar 2010 of JSPS Asia-Africa Science Platform Program
  • Place of Presentation: Quezon City, Philippines
  • Year and Date: 20110100
• [Presentation] 肺癌化学療法感受性に関わるmiRNAの探索 (2010)
  • Author(s): 鈴木俊宏、西尾和人、大森亮、兎川忠靖、櫻庭均
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20101200
• [Presentation] 制がん活性を有するアゾラト架橋白金(II)二核錯体 (2010)
  • Author(s): 米田誠治、Yuh-Lin Lingb、鈴木俊宏、植村雅子、千熊正彦
  • Organizer: 第2回メタロミクス研究フォーラム
  • Place of Presentation: 京都
  • Year and Date: 20101100