Studies on the molecular mechanisms to regulate the connection between blood vessels and lymphatic vessels

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790187
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kobe University
• Principal Investigator: DING Guo 神戸大学, 医学研究科, グローバルCOE研究員 (00514697)
• Project Period (FY): 2010 – 2011
• Keywords: 発生学 / 形態形成学

Research Abstract

We investigated the formation of venous angles that the physiology connection site between blood vessels and lymphatic vessels. Also we analyzed the abnormal blood-lymph connection in Plcg2^<-/-> mice. Valves in venous angle were consist with blood endothelial cells and lymphatic endothelial cells(LECs) two layers cells. We detected the abnormal blood-lymph connection were randomly present in Plcg2^<-/-> and LEC extends filopodia to blood vessels at connection site. In addition, platelets inhibit proliferation and migration of LECs. Taken together, We proposed new molecular mechanisms : During lymphatic development, Platelets regulate the separation between blood and lymphatic vessel by inhibiting of proliferation and migration, inducing of retraction of lymphatic endothelial cells which encounter to blood vessels during lymphatic sprouting in the peripheral tissue.

Research Products

• [Journal Article] Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells (2012)
  • Author(s): Osada M, Inoue O, Ding G, Shirai T, Ichise H, Hirayama K, Takano K, Yatomi Y, Hirashima M, Fujii H, Suzuki-Inoue K, Ozaki Y
  • Journal Title: J. Biol. Chem Volume: (in press)
  • Peer Reviewed
• [Journal Article] Essential in vivo roles of the C-type lectin receptor CLEC-2 : embryonic/neonatal lethality of CLEC-2-deficient mice by blood/lymphatic misconnections and impaired thrombus formation of CLEC-2-deficient platelets (2010)
  • Author(s): Suzuki-Inoue K, Inoue O, Ding G, Nishimura S, Hokamura K, Eto K, Kashiwagi H, Tomiyama Y, Yatomi Y, Umemura K, Shin Y, Hirashima M, Ozaki Y
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 24494-24507
  • Peer Reviewed
• [Journal Article] DOCK180 Is a Rac Activator That Regulates Cardiovascular Development by Acting Downstream of CXCR4 (2010)
  • Author(s): Sanematsu F, Hirashima M, Laurin M, Takii R, Nishikimi A, Kitajima K, Ding G, Noda M, Murata Y, Tanaka Y, Masuko S, Suda T, Meno C, Cote JF, Nagasawa T, Fukui Y
  • Journal Title: Circ Res Volume: 107(9) Pages: 1102-1105
  • Peer Reviewed
• [Presentation] Platelets contribute to the separation between blood vessels and lymphatic vessels during lymphatic sprouting (2012)
  • Author(s): Guo Ding
  • Organizer: Gordon Research Conferences(Molecular Mechanisms in Lymphatic Function & Disease)
  • Place of Presentation: Ventura Beach Marriott in Ventura CA United States
  • Year and Date: 20120300
• [Presentation] Platelets contribute to the separation between blood vessels and lymphatic vessels during lymphatic sprouting (2011)
  • Author(s): 丁国
  • Organizer: 第19回日本血管物医学会学術集会
  • Place of Presentation: 東京ステーションコンファレンス
  • Year and Date: 20111200
• [Presentation] Platelets contribute to the separation between blood vessels and lymphatic vessels during lymphatic sprouting (2011)
  • Author(s): Guo Ding
  • Organizer: The Second Pacific Symposium on Vascular Biology
  • Place of Presentation: Shilla Hetel, jeju, Korea
  • Year and Date: 20111100
• [Presentation] リンパ管の独自性を守る血小板 (2011)
  • Author(s): 丁国
  • Organizer: 第2回リンパ学に関する松本カンファランス
  • Place of Presentation: 松本市浅間温泉
  • Year and Date: 20110800
• [Presentation] Mechanisms of keeping apart blood and lymphatic vascular compartment (2010)
  • Author(s): 丁国
  • Organizer: 第18回日本血管生物医学会
  • Place of Presentation: 梅田スカイビル(大阪)
  • Year and Date: 20101200
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/vascul/index.html