2011 Fiscal Year Final Research Report
Functional relationship between reelin signal and layer formation during development of cerebral cortex
| Project/Area Number |
22790197
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Waseda University (2011)
The Institute of Physical and Chemical Research (2010) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | バイオイメージング / 大脳皮質 / 蛍光タンパク質 / リン酸化 / 可視化 / 形態形成 / 顕微鏡技術 / FRET |
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| Research Abstract |
To clarify the mechanism of laminar arrangement of neurons in the cerebral cortex, I have tried to generate the indicator of reelin signal, which controls the migration of neuron to form cortical structure in cerebrum. Since reelin signal causes phosphorylation of mouse disabled 1(mDab1), the genetically encoded indicator is consisting of yellow fluorescent protein, mDab1, SH2 domain of Src, and cyan fluorescent protein. Phosphorylation of mDab1 by sodium orthovanadate causes 7% change in the ratio of yellow to cyan emissions in live cells. Therefore, the indicator is thought to be a promising for visualizing reelin signal.
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