2011 Fiscal Year Final Research Report
Junctional membrane-targeting mechanism of cardiac L-type calcium channel
| Project/Area Number |
22790206
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | L型カルシウムチャネル / リアノジン受容体 / 心筋 |
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| Research Abstract |
In cardiac myocytes, L-type calcium channels(LTCC) form a functional signaling complex with ryanodine receptors at the junctional membrane(JM). Although the specific localization of LTCCs to JM is critical for excitation-contraction coupling, their targeting mechanism is unclear. To identify the sequences of a_<1C> responsible for the JM-targeting, we generated various mutants of aic subunit, and studied their targeting properties in dysgenic(a_<1S.-null) GLT myotubes. The results revealed that amino acids^<1681> LQAGLRTL^<1688> and^<1693> PEIRRAIS^<1700> predicted to form two adjacent a-helices in the proximal C-terminus are necessary for the JM-targeting of a_<1C>.
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