2011 Fiscal Year Final Research Report
Identification of Protein Phosphatase that Controls the Activity of Myosin Light Chain Phosphatase
| Project/Area Number |
22790240
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
SEMBA Shingo 旭川医科大学, 医学部, 助教 (40466496)
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| Project Period (FY) |
2010 – 2011
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| Keywords | シグナル伝達 / 血管平滑筋 / 一酸化窒素 / ミオシンホスファターゼ / MYPT1 |
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| Research Abstract |
In this study, we utilized affinity chromatography technique using MYPT1 fragment that was thiophosphorylated at Thr-696 (tp-MYPT) as ligand for isolation of MYPT1-phosphatases. By peptide mass fingerprinting analysis, we identified PP1cδ as one of the proteins that specifically bound to tp-MYPT but not unphosphorylated (unp)-MYPT Sepharose resin. Furthermore, overexpression of PP1cδ in smooth muscle cell line resulted the inhibition of the MYPT1 phosphorylation at Thr-696 induced by endothelin-1 sitimulation. Our results indicate that PP1cδ facilitates the dis-inhibition of MLCP activity by auto-dephosphorylating MYPT1.
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