Analysis of GATA2 gene regulation using bacterial artificial chromosome transgenic mice

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790269
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: SUZUKI Mikiko 東北大学, 大学院・医学系研究科, 助教 (80508309)
• Project Period (FY): 2010 – 2011
• Keywords: GATA2 / 大腸菌人工染色体 / トランスジェニックマウス / 3q21q26症候群 / 白血病 / 骨髄異形成症候群

Research Abstract

In this study, we demonstrated that a GATA binding element located at 77 kb upstream of Gata2 gene is an enhancer of Gata2 gene in hematopoietic progenitors using bacterial artificial chromosome(BAC)transgenic mice. The-77 enhancer is close to protooncogene EVI1 in translocated/inverted alleles between 3q21 and 3q26 in 3q21q26 syndrome. Therefore, we generated transgenic mice harboring a BAC transgene recapitulating the 3q21q26 inversion allele in order to examine contribution of the-77 enhancer to the EVI1 gene overexpression in 3q21q26 syndrome.

Research Products

• [Journal Article] Transcriptional regulation by GATA1 and GATA2 during erythropoiesis (2011)
  • Author(s): Suzuki, M., Shimizu, R. and Yamamoto, M
  • Journal Title: Int. J. Hematol Volume: 93 Pages: 150-155
  • Peer Reviewed
• [Journal Article] Genetic analysis of hierarchical regulation for Gata1 and NF-E2 p45 gene expression in megakaryopoiesis (2010)
  • Author(s): Takayama, M., Fujita, R., Suzuki, M., Okuyama, R., Aiba, S., Motohashi, H. and Yamamoto, M.
  • Journal Title: Mol. Cell. Biol. Volume: 30 Pages: 2668-2680
  • Peer Reviewed
• [Presentation] 大腸菌人工染色体を用いた新しい白血病染色体転座/逆位モデルマウスの構築 (2012)
  • Author(s): 鈴木未来子、山崎博未、清水律子、James Douglas Engel、山本雅之
  • Organizer: 造血器腫瘍研究会
  • Place of Presentation: 築地
  • Year and Date: 2012-01-28
• [Presentation] Hereditary persistence of fetal hemoglobin is associated with disruption of the HBS1L-MYB locus (2011)
  • Author(s): Suzuki M., Yamazaki H., Mukai HY., Motohashi H., Shi L., Tanabe O., Engel JD. and Yamamoto M.
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] HBS1L-MYB遺伝子間領域の変異が巨核球/赤血球運命決定異常と高胎児ヘモグロビン症を誘発する (2011)
  • Author(s): 鈴木未来子、向井陽美、山崎博未、本橋ほづみ、James Douglas Engel、山本雅之
  • Organizer: 細胞運命制御若手の会
  • Place of Presentation: 軽井沢
  • Year and Date: 2011-09-24
• [Presentation] Genome-wide analysis of GATA1 and GATA2 occupancy indicates GATA-factor switching during erythroid differentiation. (2011)
  • Author(s): Suzuki M. and Yamamoto M.
  • Organizer: Joint Minisymposium of EB Platforms and NM-GCOE
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2011-02-04
• [Presentation] Hereditary persistence of fetal hemoglobin and the HBS1L-MYB locus (2010)
  • Author(s): Suzuki M., Mukai HY., Motohashi H., Engel JD. and Yamamoto M.
  • Organizer: 5_<th> International Symposium on GATA factors
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2010-11-19
• [Presentation] A mouse model of hereditary persistence of fetal hemoglobin associated with HBS1L-MYB intergenic region (2010)
  • Author(s): Suzuki M., Mukai HY., Motohashi H., Engel JD. and Yamamoto M.
  • Organizer: The 17_<th> Hemoglobin Switching Conference
  • Place of Presentation: Oxford, UK.
  • Year and Date: 2010-09-04
• [Presentation] A novel mouse model of hereditary persistence of fetal hemoglobin associated with HBS1L-MYB intergenic region (2010)
  • Author(s): Suzuki M., Mukai HY., Motohashi H., Engel JD. and Yamamoto M.
  • Organizer: JSH International Symposium
  • Place of Presentation: Akita, Japan
  • Year and Date: 2010-07-16