2013 Fiscal Year Final Research Report
Intracellular transport and function of cancer- and neurodegenerative disease-related glycosphingolipids
| Project/Area Number |
22790280
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
YAMAUCHI Yoshio 名古屋大学, 医学(系)研究科(研究院), 講師 (00444878)
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| Project Period (FY) |
2010-10-20 – 2014-03-31
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| Keywords | スフィンゴ糖脂質 / ガングリオシド / がん / 神経変成疾患 |
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| Research Abstract |
Glycosphingolipids (GSLs) are involved in pathophysiology of cancer and neurodegenerative disorders. In this work, I studied cellular distribution and functionalities of gangliosides associated to malanoma, a skin cancer and to Niemann-Pick type C (NPC) disease, a neurodegenerative disorder. The results showed that the melanoma-associated antigen ganglioside GD3 plays a role in the activation of the regulatory factor involved in lipid biosynthesis, which promotes lipid biosynthesis for the integrity of membrane microdomains in human melanoma cells. Pharmacological inhibition of the regulatory factor prevented tumor growth. Also, I studied cellular distribution of gangliosides related to NPC disease in NPC cells. The results showed that disease-associated gangliosides accumulate in the lysosome. Several small-molecules that promote their redistribution from the lysosome to cell surface were identified.
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[Journal Article] Functional activation of Src family kinase yes protein is essential for the enhanced malignant properties of human melanoma cells expressing ganglioside GD32011
Author(s)
Hamamura K, Tsuji M, Hotta H, Ohkawa Y, Takahashi M, Shibuya H, Nakashima H, Yamauchi Y, Hashimoto N, Hattori H, Ueda M, Furukawa K, Furukawa K.
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Journal Title
J Biol Chem.
Volume: 286
Pages: 18526-18537
DOI
Peer Reviewed
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[Journal Article] GM1/GD1b/GA1 synthase expression results in the reduced cancer phenotypes with modulation of composition and raft-localization of gangliosides in a melanoma cell line2010
Author(s)
Dong Y, Ikeda K, Hamamura K,Zhang Q, Kondo Y, Matsumoto Y, Ohmi Y, Yamauchi Y, Furukawa K, Taguchi R, Furukawa K.
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Journal Title
Cancer Sci.
Volume: 101
Pages: 2039-2047
DOI
Peer Reviewed
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