Identification of reprogramming inhibitory factors by ES cell differentiation

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790285
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: OKITA Keisuke 京都大学, iPS細胞研究所, 講師 (90512434)
• Project Period (FY): 2010 – 2011
• Keywords: 人工多能性幹細胞 / iPS細胞 / 初期化 / ベクター

Research Abstract

We developed an efficient and simple method to generate integration-free human iPSCs with episomal plasmid vectors from multiple donors, including two in which only one HLA haplotype was detected. These iPSCs were effectively differentiated into various cell types in vivo and in vitro. The two putatively HLA-homozygous iPSC lines match~ 20% of the Japanese population at major HLA loci.

Research Products

• [Journal Article] A more efficient method to generate integration-free human iPS cells (2011)
  • Author(s): Okita K, et al.
  • Journal Title: Nat. Methods Volume: 8 Pages: 409-412
  • URL: http://repository.kulib.kyoto-u.ac.jp/dspace/handle/2433/139494
  • Peer Reviewed
• [Journal Article] Gingival fibroblasts as a promising source of induced pluripotent stem cells (2010)
  • Author(s): Egusa H, Okita K, et al.
  • Journal Title: PLoS One Volume: 5 Pages: e12743
  • Peer Reviewed
• [Presentation] Generation of iPS cells for therapeutic treatment (2012)
  • Author(s): Okita K
  • Organizer: Interdisciplinary approaches for the study of senescence
  • Place of Presentation: University of Cambridge, UK
  • Year and Date: 2012-02-09
• [Presentation] iPS細胞の臨床応用を目指して (2011)
  • Author(s): 沖田圭介
  • Organizer: 第21回日本サイトメトリー学会学術集会(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2011-06-25
• [Presentation] 非ウイルスベクターによる血液細胞からのヒトiPS細胞の樹立 (2011)
  • Author(s): 沖田圭介他
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] Human iPS induction from adult fibroblasts in virus free condition (2010)
  • Author(s): Okita K, et al.
  • Organizer: Biochemistry and Molecular biology 2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 人工多能性幹(iPS)細胞の課題と展望 (2010)
  • Author(s): 沖田圭介
  • Organizer: 日本放射線影響学会第53回大会(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2010-10-21
• [Book] 「分子から個体へと進化する脂質生物学」の「プロスタノイドによる免疫系・神経系制御」の章 (2012)
  • Author(s): 青井貴之、大貫茉里、沖田圭介
  • Total Pages: 210-228
  • Publisher: 羊土社
• [Book] Principles of Regenerative Medicine. Second edition, Chapter13 Induced pluripotent stem cells (2010)
  • Author(s): Okita K, Yamanaka S.
  • Total Pages: 241-252
  • Publisher: Academic press
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/index.html
• [Patent(Industrial Property Rights)] 効率的な人工多能性幹細胞の樹立方法 (2010)
  • Inventor(s): 沖田圭介/中川誠人/山中伸弥
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Industrial Property Number: 特許、EP : 10806584. 8, US : 13/389, 359, CN : 201080035155. 9, KR : 10-2012-7004663, IN : 384/KOLNP/2012, AU : 2010279913, SG : 201200686-2
  • Filing Date: 2010-08-06