Analysis of the role of deubiquitinating enzyme A20 using mouse model

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790292
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Hiroshima University
• Principal Investigator: INAGAKI Maiko 広島大学, 原爆放射線医科学研究所, 助教 (70543396)
• Project Period (FY): 2010 – 2011
• Keywords: 炎症 / がん

Research Abstract

In this study, we generated tissue-specific A20 knockout mice. Intestine epithelium-specific A20 knockout mice did not show obvious phenotype. Skin-specific A20 knockout mice showed growth retardation after weaning. We have performed historogical analysis, but we couldn't find any defect in A20 deficient skin. Instead, we found inflammation and hyperplasia on the non-glandular portions of the stomach. These data suggests A20 gene deletion in the non-glandular portions of the stomach of skin-specific A20 knockout mice, because of ectopic expression of cre. As a result, these mice might have stomach inflammation and growth reterdation.

Research Products

• [Journal Article] TAK1 (MAP3K7) signaling regulates hematopoietic stem cells through TNF-dependent and -independent mechanisms (2012)
  • Author(s): Takaesu G, Inagaki M, Takubo K, Mishina Y, Hess PR, Dean GA, Yoshimura A, Matsumoto K, Suda T, Ninomiya-Tsuji J
  • Journal Title: PLoS One Volume: 7(11) Pages: e51073
  • DOI: DOI:10.1371/journal.pone.0051073
  • Peer Reviewed
• [Journal Article] Morioka S, Inagaki M, Komatsu Y, Mishina Y, Matsumoto K, Ninomiya-Tsuji J (2012)
  • Author(s): Morioka S, Inagaki M, Komatsu Y, Mishina Y, Matsumoto K, Ninomiya-Tsuji J
  • Journal Title: Blood Volume: 120(18) Pages: 3846-57
  • DOI: DOI:10.1182/blood-2012-03-416198
  • Peer Reviewed
• [Journal Article] Epithelial transforming growth factor β-activated kinase 1 (TAK1) is activated through two independent mechanisms and regulates reactive oxygen species (2012)
  • Author(s): Omori E, Inagaki M, Mishina Y, Matsumoto K, Ninomiya-Tsuji J
  • Journal Title: Proc.Natl.Acad.Sci Volume: 109(9) Pages: 3365-70
  • DOI: DOI:10.1073/pnas.1116188109
  • Peer Reviewed
• [Journal Article] The cell adhesion gene PVRL3 is associated with congenital ocular defects (2012)
  • Author(s): Lachke SA, Higgins AW, Inagaki M, Saadi I, Xi Q, Long M, Quade BJ, Talkowski ME, Gusella JF, Fujimoto A, Robinson ML, Yang Y, Duong QT, Shapira I, Motro B, Miyoshi J, Takai Y, Morton CC, Maas RL
  • Journal Title: Hum.Genet Volume: 131(2) Pages: 235-50
  • DOI: DOI:10.1007/s00439-011-1064-z
  • Peer Reviewed
• [Presentation] Loss of Wnt5a in hematopoietic cells exacerbates Chronic Myelogenous Leukemia induced by p210BCR/ABL fusion protein (2010)
  • Author(s): Inagaki M, Koyama H, Kikuchi A, Hiroaki H
  • Organizer: Loss of Wnt5a in hematopoietic cells exacerbates Chronic Myelogenous Leukemia induced by p210BCR/ABL fusion protein
  • Place of Presentation: Osaka,Japan
  • Year and Date: 20100922-24
• [Remarks] 広島大学 原爆放射線医科学研究所 疾患モデル解析分野ホームページ
  • URL: http://home.hiroshima-u.ac.jp/sosai/top.html