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2011 Fiscal Year Final Research Report

Identificat i on of physiological roIes of a new Iipidmediator, 12-HHT

Research Project

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Project/Area Number 22790296
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionKyushu University

Principal Investigator

OKUNO Toshiaki  九州大学, 医学研究院, 助教 (60361466)

Project Period (FY) 2010 – 2011
Keywords生理活性脂質 / 受容体
Research Abstract

12-HHT is a new lipid mediator which was identified as a natural ligand for BLT2. BLT2-deficient mice exhibited severe intestinal inflammation in mice colitis model. MDCK cells transfected with BLT2 exhibited enhanced barrier function as measured by transepithelial electrical resistance(TER) and FITC-dextran leakage. Thus, BLT2 appears to protect against colitis, possibly by enhancing barrier function in epithelial cells of the colon.

  • Research Products

    (38 results)

All 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (34 results)

  • [Journal Article] A high affinity monoclonal antibody against the FLAG tag useful for Gprotein coupled receptor study2012

    • Author(s)
      Sasaki F, Okuno T, Saeki K, Lin Min, Onohara N, Kate H, Shimizu T, Yokomizo T.
    • Journal Title

      Analytical Biochemstry

      Volume: 425 Pages: 157-165

    • Peer Reviewed
  • [Journal Article] What is the natural ligand of GPR552011

    • Author(s)
      Okuno T and Yokomizo T
    • Journal Title

      Journal of Biochemistry

      Volume: 149(5) Pages: 495-497

    • Peer Reviewed
  • [Journal Article] Protective role of leukotriene B4 receptor BLT2 in murine model of inflammatory colitis2010

    • Author(s)
      Iizuka Y, Okuno T, Saeki K, Uozaki H, Okada S, Misaka T, Sate T, Ton H, Fukayama M, Takeda N, Kita Y, Shimizu T, Nakamura M, and Yokomizo T.
    • Journal Title

      Faseb J

      Volume: 24 Pages: 4678-4690

    • Peer Reviewed
  • [Journal Article] オーファンGPCRの脂質リガンド同定戦略と課題2010

    • Author(s)
      奥野利明、横溝岳彦
    • Journal Title

      実験医学増刊号

      Volume: 28 Pages: 3373-3377

  • [Presentation] Roles of BLT2 receptor in murine inflammatory colitis2012

    • Author(s)
      Okuno T. and Yokomizo T.
    • Organizer
      Keystone Symposia, Banff, Alberta, Canada
    • Year and Date
      2012-02-19
  • [Presentation] Helix 8 of leukotriene B4 receptor type 1(BLT1) inhibits LTB4-induced internalization of BLT12012

    • Author(s)
      Aratake Y, Okuno T, Yokomizo T.
    • Organizer
      Keystone Symposia, Banff, Alberta, Canada
    • Year and Date
      2012-02-18
  • [Presentation] BLT1 as a potential marker of mouse dendritic cell subset.(Oral award)2012

    • Author(s)
      Koga T., Sasaki F., Saeki K., Okuno T., Yokomizo T
    • Organizer
      The 9th Global COE International Symposium & 8th Young Investigators Forum
    • Place of Presentation
      Fukuoka
    • Year and Date
      2012-01-21
  • [Presentation] Improved immunoprecipitation method for epitopetagged GPCRs2011

    • Author(s)
      Morisaki Y., Sasaki F., Aratake Y., Okuno T., Saeki K., Yokomizo T.
    • Organizer
      The 10th JBS Biofrontier Symposium
    • Place of Presentation
      Fukuoka
    • Year and Date
      20111114-16
  • [Presentation] 12-HHT is a natural ligand for BLT22011

    • Author(s)
      Okuno T., Yokomizo T.
    • Organizer
      The 10th JBS Biofrontier Symposium
    • Place of Presentation
      Fukuoka
    • Year and Date
      20111114-16
  • [Presentation] Expression of an LTB4 receptor BLT1 in M2 macrophages2011

    • Author(s)
      Sasaki F., Koga T., Saeki K., Okuno T., Yokomizo T.
    • Organizer
      The 10th JBS Biofrontier Symposium
    • Place of Presentation
      Fukuoka
    • Year and Date
      20111114-16
  • [Presentation] Two distinct leukotriene B4 receptors : BLT1 and BLT22011

    • Author(s)
      Yokomizo T., Liu M., Saeki K., Okuno T.
    • Organizer
      The 10th JBS Biofrontier Symposium
    • Place of Presentation
      Fukuoka
    • Year and Date
      20111114-16
  • [Presentation] BLT1 as a potential marker of mouse dendritic cell subsets2011

    • Author(s)
      Koga T., Sasaki F., Saeki K., Okuno T., Yokomizo T.
    • Organizer
      The 10th JBS Biofrontier Symposium
    • Place of Presentation
      Fukuoka
    • Year and Date
      20111114-16
  • [Presentation] Two distinct pathway for biosynthesis of 12-HHT, a ligand for BLT22011

    • Author(s)
      Matsunobu T., Okuno T., Yokomizo T.
    • Organizer
      The 10th JBS Biofrontier Symposium
    • Place of Presentation
      Fukuoka
    • Year and Date
      20111114-16
  • [Presentation] Crucial role of the leukotriene B4receptor BLT2 in epidermal woundhealing2011

    • Author(s)
      佐伯和子,奥野利明,彦横溝岳
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] GPCRの免疫沈降に有用な新規抗FLAG抗体の樹立と本抗体を用いた新規GPCR結合タンパク質の探索2011

    • Author(s)
      佐々木文之,奥野利明,佐伯和子,横溝岳彦
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] つのロイコトリエンB4受容体(BLTI, BLT2)のシグナルと病態生理機能2011

    • Author(s)
      佐伯和子,劉眠,岡元冬樹,奥野利明,横溝岳彦
    • Organizer
      第84回日本生化学会大会シンポジゥム
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] ゼブラフィッシュのロイコトリエンB4/12-HHT受容体の同定2011

    • Author(s)
      奥野利明,石谷太,横溝岳彦
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] Two distinct pathways for biosynthesis of 12-HHT, a ligand for BLT22011

    • Author(s)
      松延武彦,奥野利明,横溝岳彦
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] Improved immunoprecipitation method for ePitoPe-ta99ed GPCRs2011

    • Author(s)
      森崎雄一,佐々木文之,荒武良総,奥野利明,佐伯和子,横溝岳彦
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] Helix 80f the leukotriene B4 receptor type l inhibits ligand-induced internalization2011

    • Author(s)
      荒武良総,奥野利明,横溝岳彦
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] ロイコトリエンB4第二受容体BLT2の新しいリガンドhl2-HHTの生合成機構の解明2011

    • Author(s)
      松延武彦,奥野利明,横溝岳彦
    • Organizer
      第53回日本脂質生化学会
    • Place of Presentation
      東京
    • Year and Date
      20110512-13
  • [Presentation] Roles ofl2HHTT/LTB4 receptor, BLT2 in murine inflammatory colitis2011

    • Author(s)
      Okuno T., Iizuka Y., Saeki K., Yokomizo T.
    • Organizer
      Experimental Biology
    • Place of Presentation
      Washington DC
    • Year and Date
      20110409-13
  • [Presentation] Generation of monoclonal antibodies specific for mouse leukotriene B4 receptor 1 and Nterminal FLAGsequence2011

    • Author(s)
      Sasaki F., Saeki K., Okuno T., Yokomizo T.
    • Organizer
      Experimental Biology
    • Place of Presentation
      Washington DC
    • Year and Date
      20110409-13
  • [Presentation] Toshiaki Okuno and Takehiko Yokomizo : Generation of monoclonal antibody for mouse leukotriene B_4 receptor 12011

    • Author(s)
      Fumiyuki Sasaki, Kazuko Saeki
    • Organizer
      7^<th> Global-COE International Symposium and 6^<th>' Young Investigators Forum
    • Place of Presentation
      Singapore, Feb
    • Year and Date
      20110209-14
  • [Presentation] 横溝岳彦BLT1は樹状細胞の新規マーカー分子となりうるか2011

    • Author(s)
      古賀友紹,佐伯和子,佐々木文之,奥野利明
    • Organizer
      第8回東京呼吸器リサーチフォーラム
    • Place of Presentation
      東京
    • Year and Date
      2011-11-19
  • [Presentation] Improved immunpricipitation methods for epitope-tagged GPCRs2011

    • Author(s)
      Morisaki Y, Sasaki F, Aratake Y, Okuno T, Saeki K, Yokomizo T
    • Organizer
      第6回GCOR理医連携リトリート
    • Place of Presentation
      阿蘇
    • Year and Date
      2011-08-05
  • [Presentation] Crucial role of the 12-HHT receptor BLT2 in epidermal wound healing2011

    • Author(s)
      Min Liu, Kazuko Saeki, Yoshiaki Okuno, Takehiko Yokomizo
    • Organizer
      第6回GCOR理医連携リトリート
    • Place of Presentation
      阿蘇
    • Year and Date
      2011-08-05
  • [Presentation] M2マクロファージにおけるBLTlの発現と機能解析2011

    • Author(s)
      佐々木文之、佐伯和子、奥野利明、横溝岳彦
    • Organizer
      第6回GCOR理医連携リトリート
    • Place of Presentation
      阿蘇
    • Year and Date
      2011-08-05
  • [Presentation] BLTI as a potential marker of mouse dendritic cell subsets2011

    • Author(s)
      Koga T, Sasaki F, Saeki K, Okuno T, Yokomizo T
    • Organizer
      第6回GCOR理医連携リトリート
    • Place of Presentation
      阿蘇
    • Year and Date
      2011-08-05
  • [Presentation] エイコサノイドー斉定量系の樹立とl2-HHT産生経路2011

    • Author(s)
      松延武彦、奥野利明、横溝岳彦
    • Organizer
      第6回GCOR理医連携リトリート
    • Place of Presentation
      阿蘇
    • Year and Date
      2011-08-05
  • [Presentation] Roles of leukotriene B4 receptors in inflammation and immunity2010

    • Author(s)
      Yokomizo T, Okuno T, Iizuka Y, Shimizu T
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      20101207-10
  • [Presentation] ロイコトリエンB4第一受容体BLTIのインターナリゼーションにおけるヘリックス8の役割2010

    • Author(s)
      荒武良総、奥野利明、横溝岳彦
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-10
  • [Presentation] マクロファージ貧食におけるロイコトリエンB4受容体BLTl1)の機能解析2010

    • Author(s)
      岡元冬樹、佐伯和子、奥野利明、山崎晶、横溝岳彦
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-10
  • [Presentation] マウス炎症性腸炎におけるl2-HHTT/LTB4受容体BLT2の役割2010

    • Author(s)
      奥野利明、飯塚佳子、佐伯和子、清水孝雄、中村元直、横溝岳彦
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-10
  • [Presentation] Mechanism of biosynthesis l2-HHTHHT, a natural ligand for BLT22010

    • Author(s)
      松延武彦、奥野利明、横溝岳彦
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101207-10
  • [Presentation] 炎症性腸疾患モデルから明らかになった受容体BLTl2HHTTHHT/LTB4の抗炎症作用2010

    • Author(s)
      奥野利明、飯塚佳子、佐伯和子、清水孝雄、中村元直、横溝岳彦
    • Organizer
      第7回東京リサーチフォーラム
    • Place of Presentation
      東京
    • Year and Date
      2010-11-06
  • [Presentation] Leukotriene B4 receptors in inflammation and immunity2010

    • Author(s)
      Yokomizo, T, Okuno, T, Shimizu, T
    • Organizer
      14<th> International Congress of Immunology, Kobe
    • Place of Presentation
      WS
    • Year and Date
      2010-08-27
  • [Presentation] Leukotriene B4 receptors in inflammation and immunity2010

    • Author(s)
      Yokomizo, 1, Okuno, T, Shimizu, T
    • Organizer
      14^<th> International Congress of Immunology, Kobe
    • Place of Presentation
      Poster
    • Year and Date
      2010-08-23

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Published: 2013-07-31  

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