2013 Fiscal Year Final Research Report
Investigation of the novel mechanisms regulating AML/Runx1
| Project/Area Number |
22790298
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Kyoto University (2012-2013)
Kyoto Prefectural University of Medicine (2010-2011) |
|---|
Principal Investigator |
YOKOTA Asumi 京都大学, 医学(系)研究科(研究院), 研究員 (00571556)
|
|---|
| Project Period (FY) |
2010-04-01 – 2014-03-31
|
| Keywords | Runx1 / 翻訳後修飾 / 転写因子 |
|---|
| Research Abstract |
There are nine serine/threonine residues in the C-terminal region of mouse Runx1, which are capable of being phosphorylated. We have made two Runx1 mutants; Dephospho-mimetic 1-9A and phospho-mimetic 1-9D mutant, of which all of these serine/threonine residues were mutated to alanine or aspartic acid, respectively. In luciferase assay, transcriptional activities of these mutants were affected compared with WT Runx1. However, protein expression levels or intracellular localizations were not changed.
Next we have established Runx1 mutant-KI ES cells. 1-9A or 1-9D mutant has been knocked-in to Runx1-KO ES cells. Both 1-9A and 1-9D KI ES cells could differentiate toward hematopoietic cells, indicating that these mutants retain their activity, which could rescue the differentiation blockade of Runx1 KO ES cells. As the frequencies of hematopoietic colonies were affected by these mutations, it is suggested that phosphorylation status of Runx1 might regulate its biological activity.
|
Research Products
(12 results)
-
-
-
[Presentation] Direct Interaction with Bone Marrow Mesenchymal Stromal/Stem Cells Is Required for Hematopoietic Expansion by Parathyroid Hormone. [Abstract #3689]2013
Author(s)
Yao H, Miura Y, Yoshioka S, Iwasa M, Sato A, Tamura A, Yokota A, Ashihara E, Ichinohe T, Hirai H,Maekawa T
Organizer
American Society of Hematology, 55th Annual Meeting
Place of Presentation
New Orleans, LA, USA
Year and Date
2013-12-09
-
[Presentation] Hypoxia-Adapted Myeloma Cells Possess Stem Cell Character. [Abstract #3088]2013
Author(s)
Ashihara E, Nakagawa Y, Yao H, Yokota A, Miura Y, Takata K, Kitamura Y, Hirai H , Maekawa T
Organizer
American Society of Hematology, 55th Annual Meeting
Place of Presentation
New Orleans, LA, USA
Year and Date
2013-12-08
-
[Presentation] Cytokine-STATs signalings upregulate endogenous C/EBPβ in BCR-ABL+ leukemic cells independently from BCR-ABL signaling. [Abstract #1468]2013
Author(s)
Yokota A, Hirai H, Hayashi Y, Tamura A, Sato A, Yao H, Yoshioka S, Iwasa M, Ashihara E, Miura Y, Maekawa T
Organizer
American Society of Hematology, 55th Annual Meeting
Place of Presentation
New Orleans, LA, USA
Year and Date
2013-12-07
-
[Presentation] Cell-intrinsic and -extrinsic involvement of C/EBPβ in the regulation of hematopoietic stem cells. [Abstract #1202]2013
Author(s)
Tamura A, Hirai H, Hayashi Y, Yokota A, Sato A, Yao H, Yoshioka S, Iwasa M, Ashihara E, Miura Y, Maekawa T
Organizer
American Society of Hematology, 55th Annual Meeting
Place of Presentation
New Orleans, LA, USA
Year and Date
2013-12-07
-
[Presentation] C/EBPβ expressed by bone marrow mesenchymal stromal cells is indispensable for precursor B-cell lymphopoiesis. [Abstract #1213]2013
Author(s)
Yoshioka S, Miura Y, Yao H, Iwasa M, Sato A, Tamura A, Yokota A, Hishita T, Ichinohe T, Hirai H, Maekawa T
Organizer
American Society of Hematology, 55th Annual Meeting
Place of Presentation
New Orleans, LA, USA
Year and Date
2013-12-07
-
[Presentation] Proliferation and differentiation of hematopoietic cells by osteogenic-induced bone marrow MSCs2013
Author(s)
Yao H, Miura Y, Yoshioka S, Iwasa M, Sato A, Hayashi Y, Tamura A, Yokota A, Ichinohe T, Tohyama K, Hirai H, Maekawa T
Organizer
The 75th Annual Meeting of the Japanese Society of Hematology(Jpn J Clin Hematol. 2013; 54 : 346, OS-2-113)
Place of Presentation
Sapporo
Year and Date
2013-10-12
-
[Presentation] Involvement of C/EBPβ in the regulation of hematopoietic stem cells2013
Author(s)
Tamura A, Hirai H, Hayashi Y, Yokota A, Yao H, Yoshioka S, Sato A, Iwasa M, Ashihara E, Miura Y, and Maekawa T.
Organizer
The 75th Annual Meeting of the Japanese Society of Hematology(Jpn J Clin Hematol. 2013; 54 : 437, PS-1-2)
Place of Presentation
Sapporo
Year and Date
2013-10-11
-
-
-
