2011 Fiscal Year Final Research Report

Molecular mechanism of neurite outgrowth regulated by PLCdelta3

Research Project

Project/Area Number	22790300
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	General medical chemistry
Research Institution	Institute for Developmental Research, Aichi Human Service Center (2011) Tokyo University of Pharmacy and Life Science (2010)
Principal Investigator	KOUCHI Zen 愛知県心身障害者コロニー発達障害研究所, 病理学部, 研究員 (70322485)
Project Period (FY)	2010 – 2011
Keywords	細胞内シグナル伝達 / ホスホリパーゼC
Research Abstract	Phospholipase Cδ3(PLCδ3), which is highly expressed in neuronal tissues, hydrolyzes phosphatidylinositol 4, 5-bisphosphate(PI(4, 5) P_2) and generates two second messengers, i. e. IP_3 and DAG. PLCδ3 knockdown in cerebral cortex of E14 embryo by in utero electroporation caused the migratory inhibition of cortical neurons in developing cerebral cortical plate. Furthermore, we demonstrated that neuronal outgrowth was inhibited in cerebral neurons, cerebellar granule cells, or Neuro2a cells when PLCδ3 was transiently knocked down during differentiation.