2011 Fiscal Year Final Research Report
Identification of the genes involved in the tumorigenesis and spontaneous regression of neuroblastoma via array CGH analysis with model mice
| Project/Area Number |
22790311
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
KISHIDA Satoshi 名古屋大学, 大学院・医学系研究科, 助教 (20402563)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 神経芽腫 / モデルマウス / CGHアレイ / Cited2 / がん幹細胞 |
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| Research Abstract |
We tried to identify the genes involved in the tumorigenesis and spontaneous regression of neuroblastoma via array CGH analysis with MYCN Tg mice, a model for neuroblastoma. For the purpose of evaluating the huge number of chromosomal aberrations, we set three parameters and calculated the scores. As a result, we identified the gene, Cited2, which is deleted in all 12 terminal tumors. Neuroblastoma in MYCN Tg mice turned out to be composed of two distinguishable populations in terms of the expression level of Cited2. The cells with low Cited2 expression showed strikingly higher tumorigenic ability compared to high ones. We also found that Cited2 affected the expression of other neuroblastoma-related transcription factors. In addition, although the cells with low Cited2 expression gave rise to high ones in vivo, the opposite never occurred. This result indicates the hierarchy that the low ones are superior to high ones. Taken together, the low expression of Cited2 might be the hallmark as cancer stem cells of neuroblastoma.
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