Molecular mechanism of cancer cells invasiveness regulated by Ror2 signaling in human osteosarcoma cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790315
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Kobe University
• Principal Investigator: YAMAGATA Kaoru 神戸大学, 大学院・医学研究科, グローバルCOE研究員 (80533786)
• Project Period (FY): 2010 – 2011
• Keywords: Wnt5a / Ror2 / Dvl2 / AP1 / MMP-13 / 軟骨内骨化

Research Abstract

Constitutively active Wnt5a-Ror2 signaling results in activated Dishevelled2, Rac1, JNK and transcriptional factor AP1 in human osteosarcoma SaOS-2 cells, resulting in subsequent induced expression of MMP-13 that is capable of degrading extracellular matrix, involved in cancer cell invasiveness. Wnt5a-Ror2 signaling involves endochondral ossification of cartilaginous tissue in mouse embryo, indicating crucial role in developmental conditions.

Research Products

• [Journal Article] Dissection of Wnt5a-Ror2 signaling leading to matrix metalloproteinase(MMP)-13 expression (2012)
  • Author(s): K. Yamagata, X. Li, S. Ikegaki, C. Oneyama, M. Okada, M. Nishita, Y. Minami.
  • Journal Title: J. Biol. Chem Volume: 287(2) Pages: 1588-99
  • Peer Reviewed
• [Presentation] Dissection of Wnt5a-Ror2 signaling leading to matrix metalloproteinase(MMP)-13 expression (2011)
  • Author(s): 山形薫、池垣俊吉、李欣、南康博
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Dissection of Wnt5a-Ror2 signaling leading to matrix metalloproteinase(MMP)-13 expression (2011)
  • Author(s): 山形薫、池垣俊吉、李欣、南康博
  • Organizer: Joint Symposium on Integrative Membrane Biology and Signal Transduction Medicine
  • Place of Presentation: University of Washington-Kobe University神戸
  • Year and Date: 2011-12-13
• [Presentation] Molecular analysis of MMP13 gene expression by Wnt5a-Ror2 signaling in human osteosarcoma cells (2011)
  • Author(s): 山形薫、池垣俊吉、李欣、南康博
  • Organizer: 第3回次世代シグナル伝達医学グローバルCOE研究討論会
  • Place of Presentation: 淡路
  • Year and Date: 2011-07-11
• [Presentation] がんの進展におけるWnt5a-Rorシグナルの機能解析 (2011)
  • Author(s): 山形薫、南康博
  • Organizer: 第3回シグナルネットワーク研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-27
• [Presentation] Analysis of MMP13 gene expression induced by Wnt5a-Ror2 signaling in human osteosarcoma cell line (2010)
  • Author(s): 山形薫、李欣、渡邊康秀、南康博
  • Organizer: 第2回次世代シグナル伝達医学グローバルCOE研究討論会
  • Place of Presentation: 淡路
  • Year and Date: 2010-07-05
• [Presentation] Wnt5a-Ror2シグナルによる細胞極性・移動制御とがんの浸潤 (2010)
  • Author(s): 南康博、山形薫、西田満
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-20
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/medzoo/